Epigenetic Integrity of Spermatozoa in Patients With Germinal Testicular Tumours

Epigenetic Integrity of Spermatozoa in Patients With Germinal Testicular Tumours: Potential Risks and Consequences for the Conceptus

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT03262207

Acronym

GAMETH

Enrollment

Registered

2017-08-25

Start date

2016-09-30

Completion date

2017-09-30

Last updated

2017-08-25

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Seminomas

Brief summary

Recent data suggest that sperm cells carry an epigenetic message during spermatogenesis and that this message is crucial for the future development of the embryo. This epigenetic signature is notably represented by methylation of genes subjected to imprinting (GSI) and the methylation of transposable elements (TE). Data on the maintenance of the imprint and of the control of TE accompanying human gametogenesis in a context of adult germinal testicular cancers, seminomas, are extremely fragmentary for tumour tissues and inexistent for gametes. The aim of this study is to determine whether patients with seminomas in comparison with fertile men carry a higher risk of presenting epigenetic alterations affecting their gametes. This study is based on the use of an existing collection of biological samples. 90 samples will be selected and split into 3 groups: * Group 1: 30 sperm samples from patients with seminomatous testicular tumours * Group 2: 30 sperm samples from fertile patients * Group 3: 30 sperm samples from infertile patients After treatment of the samples (thawing, cell sorting and removal of cryoprotectants), they will be analysed.

Interventions

OTHERExtraction genomic DNA

OTHERAnalysis of methylation profiles of numerous GSI and TE

OTHERAnalysis of methylation for the whole genome (sub-population of 15 samples from group 1)

OTHERMethod of protein detection by immunostaining and flow cytometry

Study design

Observational model

OTHER

Time perspective

OTHER

Eligibility

Sex/Gender

MALE

Age

18 Years to No maximum

Healthy volunteers

Yes

Inclusion criteria

* biological samples from persons who have provided written consent * biological samples of patients over 18 years old * with testicular tumours/Fertile/Infertile * Matched for age with patients suffering from testicular tumours

Exclusion criteria

* biological samples of persons without national health insurance cover * biological samples from patients with chronic hepatitis B or C or HIV infection

Design outcomes

Primary

Measure	Time frame
Analysis of methylation profiles in paternal genes subjected to imprinting (GSI)	at baseline

Countries

France

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026