Pulmonary Disease, Chronic Obstructive
Conditions
Brief summary
Hypothesis: 1. Innate immunity is altered in certain patients with COPD and frequent exacerbations, a fact that makes them more susceptible to being infected by bacteria. 2. The electronic nose is able to detect patterns of specific VOCs for exacerbations of infectious origin.
Detailed description
Bronchial infection has been described as the leading cause of COPD exacerbations. Different studies with invasive endoscopic techniques have demonstrated the presence of bacteria in the air in 40-70% of exacerbations of the disease. In addition, these patients have a higher concentration of cells and proinflammatory cytokines in the airway. This increased inflammation is associated with more frequent and more severe exacerbations, which worsen this vicious circle. It is not known why some patients with COPD are more susceptible than others to bronchial, acute or chronic infection. Recent studies have suggested the importance of lung innate immunity, both humoral (proteins with antibiotic activity, inflammatory mediators) and cell (neutrophils, macrophages) as the key to the defense of the lung against infectious agents external factor. There may be a bidirectional relationship between immune response and bronchial infection in COPD exacerbations. Te main objectives of our study are: 1. To study the expression of mucin, PAM and TLR in the airway of patients with COPD and frequent exacerbations (FE) and its relationship with the infection of the airway. 2. Determine the patterns of volatile organic compounds (VOCs) detected by electronic nose associated with bronchial infection in patients with COPD and FE. Secondary objectives: 1. To study the relationship between the expression of mucin, PAM and TLR with pulmonary and systemic inflammation. 2. To study the relationship between the expression of mucin, PAM and TLR with bronchial bacterial load. 3. To study the expression of mucin, PAM and TLR at the time of COPD exacerbations and subsequent clinical phase stability. 4. Determine VOC patterns for specific pathogens (H. influenzae, S. pneumoniae, P. aeurginosa). 5. To study the time evolution of patterns of VOCs after a COPD exacerbation.
Interventions
None listed
Sponsors
Fundació Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
45 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
1. Diagnosis of COPD according to national and international guidelines. 2. Presence of ≥ 2 exacerbations requiring admission in the last 12 months. 3. Signature of informed consent.
Exclusion criteria
1. Presence of other lung diseases 2. terminal concomitant disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Relationship between infection and airway innate immunity of patients with COPD and frequent exacerbations. | 6 months | Mucine levels will be determined with ELISA kits |
| Association between volatile organic compounds (VOCs) detected by an electronic nose and bronchial infection in patients with COPD and frequent exacerbations. | 6 months | The patterns of specific volatile organic compounds in echaled air will be determined with electronic nose device. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Relationship between airway innate immunity and systemic inflammation. | 6 months | Mucin levels will be determined with ELISA kits |
| Relationship between airway innate immunity and bronchial bacterial load. | 6 months | Mucin levels will be determined with ELISA kits. |
Countries
Spain
Contacts
Primary ContactOriol Sibila, PhD
Outcome results
None listed