Human Milk Oligosaccharides, Allergic Manifestation
Conditions
Keywords
Allergic manifestation, Human milk oligosaccharides, Breast Milk
Brief summary
Compare the HMO composition in breast milk of infants with allergic manifestation (atopic dermatitis and food allergy) in the first 3 years of life, healthy infants with allergic mother and healthy infants with non-allergic mother.
Detailed description
* Parents of breast-fed infant from general pediatric clinic and allergy clinic of King Chulalongkorn Memorial Hospital were approached for informed about the research and ask to join this project * After completed informed consent, demographic data was collected and filled in case record form * Milk of mother in both allergic and control groups were collected 60 ml then transferred to freeze at -80oc to for human milk oligosaccharide analysis
Interventions
PROCEDUREmilk collection
* Parents of breast-fed infant from general pediatric clinic and allergy clinic of King Chulalongkorn Memorial Hospital were approached for informed about the research and ask to join this project * After completed informed consent, demographic data was collected and filled in case record form * Milk of mother in both allergic and control groups were collected 60 ml then transferred to freeze at -80oc to for human milk oligosaccharide analysis Method of human milk oligosaccharide analysis : 1. Ultra High Pressure Liquid Chromatography(UHPLC) with fluorescence method 2. High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD) method
Sponsors
Chulalongkorn University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
No minimum to 2 Years
Healthy volunteers
Yes
Inclusion criteria
1. mothers with breast-fed infant with or without allergic manifestation 2. Intend to participate for follow up and sign consent to participate in the study
Exclusion criteria
1. Mothers with any medical illness or high risk infant pregnant 2. Infants with disease which affected allergic disease 3. No inform consent 4. Anything in the opinion of the investigator that would prevent children from completing the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Atopic dermatitis | first 2 years of life | Relapsing eczematous typical morphology on typical distribution (Scalp, face, neck, and extensor surfaces of the skin) Typical morphology Acute and subacute : erythematous papules and plaques, may vesiculate and become exudative Chronic : lichenification, papules and excoriations |
| IgE mediated food allergy | first 2 years of life | confirmed by oral food challenge |
| Non-IgE mediated food allergy | first 2 years of life | Presentation of clinical ex. bloody stool when repeating episode occurred with the same food more than twice. Improvement of symptom after eliminating of suspected food |
Countries
Thailand
Contacts
Primary ContactNarissara Suratannon, MD
Backup ContactJintana Chataroopwijit, MD
Outcome results
None listed