Phenotypic Characterization of Alveolar Macrophages 2016 (Caract-Aires-2016)

Phenotypic Characterization of Alveolar Macrophages 2016

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT03250897

Acronym

Caract-Aires

Enrollment

Registered

2017-08-16

Start date

2016-11-01

Completion date

2017-12-01

Last updated

2026-06-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Lung Diseases

Keywords

Alveolar macrophages, Blood monocyte-derived macrophages, FACS analysis, Broncho-alveolar lavage fluids, Non-infectious lung diseases

Brief summary

The aim of the study is to investigate by flow cytometry the phenotype of alveolar macrophages (AMs) isolated from patients with non-infectious lung diseases.

Detailed description

Although alveolar macrophages (AMs) represent the main immune cell population in the human lungs, consensus on their phenotypic characterization is still missing. Current translational protocols use human AMs obtained by invasive methods (broncho-alveolar lavage and lung biopsy) or differentiate macrophages from blood monocytes cultured in the presence of growth factors such as macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF). The group has already published the phenotypic and functional comparison of human blood monocyte-derived macrophages differentiated with M-CSF or GM-CSF. Aim of this study is to use a multi-staining flow cytometry (FACS analysis) to better characterize the phenotype of AMs isolated from broncho-alveolar lavage fluids of patients affected by non-infectious lung diseases. The FACS profiles of AMs will be further compared to the phenotypic profile of blood monocyte-derived macrophages differentiated in the presence of GM-CSF or M-CSF. The results of this study will allow to define the most valid in vitro model of AMs in a non-infectious setting.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

Inclusion criteria

* Patients affected by non-infectious lung diseases and for whom a broncho-alveolar lavage is scheduled for their medical care in the pulmonary division * Patient Information leaflet provided * Written informed consent of the patient

Exclusion criteria

* A suspected respiratory infection

Design outcomes

Primary

Measure	Time frame	Description
To investigate by flow cytometry the phenotype of alveolar macrophages (AMs) isolated from broncho-alveolar lavage fluids of patients with non-infectious lung diseases.	1 year	Characterization of membrane proteins by multi-staining flow cytometry (FACS analysis)

Secondary

Measure	Time frame
To compare the phenotypic profile of AMs from broncho-alveolar lavage fluids of patients with non-infectious lung diseases to that of blood monocyte-derived macrophages differentiated in the presence of GM-CSF or M-CSF and obtained from healthy donors	1 year

Countries

France

Contacts

PRINCIPAL_INVESTIGATORStéphane Jouneau, Professor

Rennes University Hospital

Outcome results