Osteoclast Impairment in Chronic Periodontitis by Regenerative Materials.

Platelet Rich Fibrin/Biphasic Calcium Phosphate Impairs Osteoclasts Differentiation and Promotes Apoptosis by the Intrinsic Mitochondrial Pathway in Chronic Periodontitis

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT03227367

Enrollment

Registered

2017-07-24

Start date

2014-03-17

Completion date

2017-03-18

Last updated

2017-07-26

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Osteoclastic Bone Loss, Periodontal Bone Loss, Periodontal Disease

Keywords

biomaterials, apoptosis, bone regeneration

Brief summary

The present study is an effort to investigate the hypothesis that Platelet Rich Fibrin/Biphasic Calcium Phosphate may play a role in inhibiting osteoclasts differentiation and in bone loss.

Detailed description

Study design Fifty-five individuals from a suburban population were recruited. Out of them, 5 individuals refused to participate in the study. Finally, 50 participants were selected and divided into two groups, control group \[Healthy individuals\]: 25 Males/Females, aged 25 to 35 years and test group \[Chronic periodontitis patients\]: 25 Males/Females aged 30 to 45 years. Demographic variables and periodontal parameters were measured in both the groups.The investigators explored the effect of Platelet rich fibrin (PRF)/Biphasic calcium phosphate (BCP) on differentiation and survival of osteoclasts obtained from peripheral blood of Chronic Periodontitis patients.

Interventions

OTHERother : PRF

mononuclear cells from the peripheral blood of chronic periodontitis patients were intervened with PRF, BCP and PRF/BCP combination

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

OTHER

Masking

NONE

Intervention model description

Control group: (Healthy individuals) comprised of 25 participants with clinically healthy periodontium. Test group: (Chronic periodontitis patients) comprised of 25 participants with signs of clinical inflammation and bone loss.

Eligibility

Sex/Gender

ALL

Age

25 Years to 35 Years

Healthy volunteers

Yes

Inclusion criteria

(Control group) * Subjects with age group between 30 to 45 years. * Non-smokers. * No previous history of periodontal treatment. Inclusion Criteria: (Test group) * Subjects with age group between 30 to 45 years. * Presence of atleast 20 teeth * More than 30% of the sites with clinical attachment loss. * Non-smokers. * No previous history of periodontal treatment.

Exclusion criteria

( both the groups) * Individuals with systemic disease * Subjects under antibiotics or anti-inflammatory drugs within the previous 3 months. * Pregnant and lactating women. * Allergic to antibiotics.

Design outcomes

Primary

Measure	Time frame	Description
probing pocket depth	base line	probing pocket dept was measured using Williams periodontal probe at base line

Secondary

Measure	Time frame	Description
Protein expression on osteoclast cells was estimated with BCP, PRF alone and BCP/PRF combination.	24 hours from baseline	Protein expression on cells was analyzed using Western blot in all the groups
The activity of caspase-3 in OCs.	24 hours from base line	The activity of caspase-3 was seen in OCs in all the groups

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026