Overweight and Obesity, Type 2 Diabetes, Bariatric Surgery, Gut Hormones, Dietary Fat
Conditions
Brief summary
To investigate the effects of different fat metabolites on gut hormone secretion in gastric bypass operated subjects and BMI-matched controls.
Detailed description
Dietary triglycerides are hydrolyzed during digestion to two fatty acids and one 2-monoacyl glycerol. The study will investigate the effects of these different fat components on gut hormone secretion in gastric bypass operated patients and non-operated BMI-matched controls.
Interventions
DIETARY_SUPPLEMENTC-8 dietary oil
Ingestion of 13.15 g C-8 dietary oil providing 0.0216 mol of triglycerides.
DIETARY_SUPPLEMENTTricaprylin
Ingestion of 10.17 g tricaprylin providing 0.0216 mol of triglycerides.
DIETARY_SUPPLEMENTOlive oil
Ingestion of 19 g olive oil providing 0.0216 mol of triglycerides.
Sponsors
University of Copenhagen
Hvidovre University Hospital
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
Gastric bypass operated patients: Inclusion Criteria: * Uncomplicated gastric bypass surgery performed minimum 12 months prior to study
Exclusion criteria
* Type 1 or 2 diabetes mellitus prior to or after gastric bypass surgery * Pregnancy or breastfeeding * Haemoglobin levels below 6,5 mM * Gall bladder removal Healthy control subjects:
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Within group difference in glucagon-like peptide-1 (GLP-1) secretion (evaluated by AUC above basal). | 0-240 min following fat ingestion. | Comparison of the GLP-1 responses (evaluated by AUC above basal) induced by the different oils. |
| Within group difference in Gastric Inhibitory Peptide (GIP) secretion (evaluated by AUC above basal). | 0-240 min following fat ingestion. | Comparison of the GIP responses (evaluated by AUC above basal) induced by the different oils. |
| Within group difference in cholecystokinin (CCK) secretion (evaluated by AUC above basal). | 0-240 min following fat ingestion. | Comparison of the CCK responses (evaluated by AUC above basal) induced by the different oils. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Responses (evaluated by AUC above basal) of peptide YY (PYY) within and between groups. | 0-240 min following fat ingestion. | — |
| Responses (evaluated by AUC above basal) of oxyntomodulin within and between groups. | 0-240 min following fat ingestion. | — |
| Between group comparison of GLP-1 secretion (evaluated by AUC above basal). | 0-240 min following fat ingestion. | GLP-1 secretion (evaluated by AUC above basal) in gastric bypass operated patients compared to BMI-matched control subjects for each of the oils. |
| Between group comparison of GIP secretion (evaluated by AUC above basal). | 0-240 min following fat ingestion. | GIP secretion (evaluated by AUC above basal) in gastric bypass operated patients compared to BMI-matched control subjects for each of the oils. |
| Between group comparison of CCK secretion (evaluated by AUC above basal). | 0-240 min following fat ingestion. | GIP secretion (evaluated by AUC above basal) in gastric bypass operated patients compared to BMI-matched control subjects for each of the oils. |
| Responses (evaluated by AUC above basal) of insulin within and between groups. | 0-240 min following fat ingestion. | — |
| Responses (evaluated by AUC above basal) of glucagon within and between groups. | 0-240 min following fat ingestion. | — |
| Responses (evaluated by AUC above basal) of lipid metabolites (triglyceride, cholesterol, fatty acids) within and between groups. | 0-240 min following fat ingestion. | — |
| Responses (evaluated by AUC above basal) of neurotensin within and between groups. | 0-240 min following fat ingestion. | — |
| Responses (evaluated by AUC above basal) of glucose within and between groups. | 0-240 min following fat ingestion. | — |
| Between group comparison of differences in GLP-1 secretion (evaluated by AUC above basal). | 0-240 min following fat ingestion. | Differences in GLP-1 secretion (evaluated by AUC above basal) induced by the different oils in gastric bypass operated patients compared with differences in BMI-matched control subjects. |
| Between group comparison of differences in GIP secretion (evaluated by AUC above basal). | 0-240 min following fat ingestion. | Differences in GIP secretion (evaluated by AUC above basal) induced by the different oils in gastric bypass operated patients compared with differences in BMI-matched control subjects. |
| Between group comparison of differences in CCK secretion (evaluated by AUC above basal). | 0-240 min following fat ingestion. | Differences in CCK secretion (evaluated by AUC above basal) induced by the different oils in gastric bypass operated patients compared with differences in BMI-matched control subjects. |
| Responses (evaluated by AUC above basal) of fibroblast growth factor 19 (FGF-19) within and between groups. | 0-240 min following fat ingestion. | — |
| Responses (evaluated by AUC above basal) of bile acids within and between groups. | 0-240 min following fat ingestion. | — |
| Responses (evaluated by AUC above basal) of C-peptide within and between groups. | 0-240 min following fat ingestion. | — |
Countries
Denmark
Contacts
Primary ContactSten Madsbad, Professor
Outcome results
None listed