Diabetes Mellitus, Type 2
Conditions
Keywords
lipolysis, adipose tissue, exercise
Brief summary
The aim of this study is to investigate whether metformin has inhibitory effect on exercise-induced lipolysis in subcutaneous adipose tissue in healthy lean man. Ten healthy lean mean will follow protocols consisting of 60 minutes of exercise on bicycle ergometer combined with a) orally administered metformin and b) locally administered metformin into adipose tissue and c) without metformin treatment. Microdialysis is used for assessment of metabolites in situ in subcutaneous adipose tissue.
Detailed description
Metformin and physical activity are often recommended as the first therapeutic agents to improve glycemic control for prediabetes and type 2 diabetes (ADA). It has been reported that metformin decrease plasma concentration of non-esterified fatty acids in patients with type 2 diabetes which might be associated with the inhibition of lipolysis in adipose tissue. However, the inhibition of lipolysis during physical exercise, would not be probably beneficial. Thus, the effect of metformin on lipolysis during exercise is investigated in this protocol. As the lipolysis regulation is often impaired in obese and diabetic patients, the healthy lean men were chosen for this pilot study. Glycerol will be analyzed as a measure of lipolytic rate in adipose tissue and in plasma. Lactate levels will be observed. Our findings should provide a new background about the combination of physical activity and metformin medication.
Interventions
DRUGMetformin Oral
DRUGMetformin local in AT
OTHERExercise
Sponsors
Charles University, Czech Republic
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
25 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy lean men, non obese, BMI 20-28 kg/m2
Exclusion criteria
* weight change within the 3 months before the study, * hypertension, * impaired fasting glucose * diabetes * hyperlipidemia * drug or alcohol abuse * smoking
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in adipose tissue lipolysis in response to exercise | Minute -60, 15, 30, 45, 60 and 120 from the start of exercise | Glycerol concentration in dialysate outcoming from adipose tissue and in plasma - kinetic study |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in lactate production in response to exercise | Minute -60, 15, 30, 45, 60 and 120 from the start of exercise | Concentration of lactate in dialysate and in plasma - kinetic study |
Countries
Czechia
Outcome results
None listed