Asthma, Pulmonary Disease, Chronic Obstructive, Smoking
Conditions
Keywords
Bronchial epithelial cell, mucus, Air Liquid Interface
Brief summary
This is a prospective study comparing 4 groups: (1) non-smoking controls, (2) smokers without chronic obstructive pulmonary disease (COPD), (3) smokers with COPD, (4) severe asthma. Bronchial biopsy specimens from each subject will be obtained to produce air-liquid-interface cell cultures. These will then be used to make observations concerning cilia and mucus rheology. This is a first pilot study. The working hypothesis is that the largest group differences will be found for cilia densities; the latter metric was thus chosen as a primary criterion.
Interventions
PROCEDUREBronchial Biopsy
Bronchial biopsies will be performed on all subjects.
OTHERALI culture
Air-liquid-interface cell cultures will be derived from bronchial biopsy specimens. We are expecting several replicate cell lines from each biopsy specimen.
Sponsors
University Hospital, Montpellier
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
for non smoking controls: * The subject has given free and informed consent and signed the consent form * The subject is affiliated with or beneficiary of a medical insurance program * The subject has never smoked * No obstructive breathing disorder (forced expiratory volume at one second (FEV1) \>70% of predicted value; the ratio of FEV1 to forced vital capacity (FEV1/FVC) is \> 0.7) * No known respiratory disease Inclusion criteria for smokers without COPD: * The subject has given free and informed consent and signed the consent form * The subject is affiliated with or beneficiary of a medical insurance program * Smoker or former smoker (\>= 30 pack years) * No obstructive breathing disorder (forced expiratory volume at one second (FEV1) \>70% of predicted value; the ratio of FEV1 to forced vital capacity (FEV1/FVC) is \> 0.7) Inclusion criteria for smokers with COPD: * The subject has given free and informed consent and signed the consent form * The subject is affiliated with or beneficiary of a medical insurance program * Smoker or former smoker (\>= 30 pack years) * Subject presenting with COPD: FEV1/FVC \< 0.7 (based on spirometry values in the medical file and performed in the 3 months prior to inclusion) * The subject requires bronchial fibroscopy (decision made by pneumologist) * COPD of grade 2 or 3 according to GOLD criteria (Global Initiative for Chronic Obstructive Lung Disease) Inclusion criteria for subjects with severe asthma: * The subject has given free and informed consent and signed the consent form * The subject is affiliated with or beneficiary of a medical insurance program * Non-smoker or former smoker (\<10 pack years) * Subject presenting with severe asthma: FEV1/FVC \>= 0.7 and FEV1 \< 80% * The subjects requires bronchial fibroscopy (decision made by pneumologist)
Exclusion criteria
* The subject has an extensive neoplastic disease * The subject has another active lung disease (tuberculosis, pulmonary interstitium disease, active or recent pulmonary infection) * The subject has had a recent psychiatric disorder (within one year prior to inclusion and documented by a specialist consultation) * Emergency situations (object extractions...) * Subject consuming illicit drugs or alcohol * The subject is in an exclusion period determined by a previous study * The subject is under judicial protection, or is an adult under any kind of guardianship * It is impossible to correctly inform the patient * The subject cannot fluently read French
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Surface density of active cilia | Day 0 + approximately 2 months of cell culture time | Surface density of active cilia |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Cilia beating frequency | Day 0 + approximately 2 months of cell culture time | The frequency at which cilia beat. |
| Mucus viscosity | Day 0 + approximately 2 months of cell culture time | The viscosity of mucus produced by cell cultures. |
| Mucus elasticity | Day 0 + approximately 2 months of cell culture time | The elasticity of mucus produced by cell cultures. |
| Cilia beat orientation | Day 0 + approximately 2 months of cell culture time | The direction in which cilia beat |
Countries
France
Outcome results
None listed