Exercise-induced Oxidative Stress
Conditions
Keywords
Exercise, Oxidative stress, Antioxidant
Brief summary
The aim of this study is to assess the antioxidant capacity of two mango base beverages (AM1, AM2), against exercise-induced oxidative stress.
Detailed description
Introduction. The post-consumption physiological effect of mango cv. Ataulfo pulp (MP) and peel (MC) in humans before exercise has been poorly evaluated. Objective. To evaluate the physiological response, to consumption of two mango base beverages, AM1 (200g MP/ 600mL water) and AM2 (160g MP; 40g MC / 600mL water), before and after a submaximal exercise test. Methods. The antioxidant profile and physicochemical characteristics of AM1 and AM2 were evaluated. Several cardiorespiratory, anthropometric and body composition parameters of 19 university students, prior to the consumption of AM1, AM2 and water, were evaluated in three non-consecutive sessions (self-control cases). The glycemic, lactic and antioxidant (FRAP), uric acid (AU), reduced glutathione (GSH) and lipid oxidation (TBARS) and protein carbonyls (PC) responses were evaluated in plasma before and after an incremental cycle ergometer exercise test.
Interventions
DIETARY_SUPPLEMENTMango pulp beverage
The subject consumes mango pulp beverage (200 g mango pulp + 600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.
DIETARY_SUPPLEMENTMango pulp and peel beverage
The subject consumes mango pulp and peel beverage (160 g mango pulp + 40 g mango peel + 600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.
DIETARY_SUPPLEMENTControl beverage
The subject consumes control beverage (600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.
Sponsors
Universidad Autonoma de Ciudad Juarez
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
NONE
Intervention model description
The prescription of the treatments (AM1, AM2) and control (water) was randomized. The subject consumed 600mL of AM1, AM2 or water. 2 hours later subject performed the exercise test. Next treatment was evaluated after a week of wash-out.
Eligibility
Sex/Gender
MALE
Age
17 Years to 28 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy male * university student * no alcohol, cigar or drugs consumption
Exclusion criteria
* no antioxidant supplement or ergogenic consumption * health issues with performing physical activity
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Antioxidant capacity change | 0 min (before beverage intake), 9-15 min (immediately after exercise test) | Assessed by FRAP change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Oxidative stress change | 0 min (before beverage intake), 9-15 (immediately after exercise test) | Assessed by TBARS concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis. |
| Lactic acid concentration change | 0 min (before performing exercise test), 3 min, 6 min, 9 min, 12 min, 15 min (during exercise test) | Assessed by lactic acid concentration change. Capillary blood samples were taken from the fingertip, using a lancet and analyzed (YSI modelo 1500, Yellow Springs, USA) |
| Postprandial glycemic change | 0 min (before beverage intake), 30 min, 60 min, 90 min, 120 min (after beverage intake) | Assessed by blood glucose concentration change. Capillary blood samples were taken from the fingertip, using a lancet, and analyzed (ReliOn Confirm/micro Test Strips ARKRAY, USA) |
Outcome results
None listed