Coronary Artery Disease, Diabetes Mellitus, Mitochondrial Metabolism Disorders
Conditions
Brief summary
Cardiac ischemia is a common pathological condition, known to elicit multiple pathological processes at the cellular level. One of the most affected is thought to be cellular metabolism, key for the adequate cardiac function. The aim is to study mitochondrial bioenergetic function, interaction with other cellular systems and influence of several co-morbidities in myocardium of the affected patients.
Detailed description
Coronary artery disease, one of the most common pathologies in the developed world, causes hypoperfusion of myocardial tissue, usually evident by the presence of anginal pain. This myocardial ischemia elicits alterations in normal cardiomyocyte physiology, which gradually deteriorate cellular function, affecting the performance of the entire organ. The condition is frequently further complicated (and aggravated) by the presence of various co-morbidities, such as diabetes mellitus. The primary aim of this study is to investigate the cardiomyocyte bioenergetics and the consequences of potentially reduced mitochondrial metabolic function in ischemic heart, and evaluate the potential contribution of other conditions, primarily ones affecting metabolic homeostasis.
Interventions
OTHERStandard therapy
Patients with therapy prescribed independent of the study
Sponsors
University of Split, School of Medicine
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
50 Years to 85 Years
Healthy volunteers
No
Inclusion criteria
* Coronary artery disease * Indication for coronary artery bypass grafting surgery
Exclusion criteria
* Emergency patients * Patients with LV ejection fraction (LVEF) below 30% * Patients with severe renal, hepatic or pulmonary disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Mitochondrial oxygen consumption (rate of oxygen consumption per milligram of left ventricular tissue) | 2016-2020 | Mitochondrial respiration in nmol O2/minute/mg of tissue weight will be measured as an indicator of mitochondrial ATP production capacity, using different metabolic substrates |
| Myocardial production of reactive oxygen species | 2016-2020 | Myocardial production of reactive oxygen species will be measured using electron paramagnetic resonance and fluorometry |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Anti-cardiolipin antibodies in the blood of patients with symptoms of cardiac ischemia | 2016-2020 | Anticardiolipin antibodies assessed in venous blood of patients with angina pectoris undergoing coronary angiography |
Countries
Croatia
Outcome results
None listed