Differential Diagnostics of Etiology of Acute Infections

Differential Diagnostics of Etiology of Acute Infections Based on the Analysis of Expression of Activation Markers on the Surface of Peripheral Blood Monocytes and Neutrophils.

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT03161951

Enrollment

169

Registered

2017-05-22

Start date

2017-01-03

Completion date

2018-12-29

Last updated

2019-01-09

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Intestinal Infectious Diseases, Neuroinfections

Keywords

acute Infections, flow cytometry, cell activation

Brief summary

Method for diagnostics of the origin of infections (bacterial vs viral) based on the identification of activation markers of blood neutrophils and monocytes will be developed.

Detailed description

During the study, method for diagnostics of the origin of infections (bacterial vs viral) based on the identification of activation markers of blood neutrophils and monocytes will be developed.This method will allow to make a diagnosis within an hour after blood sampling. The expression of the following markers of activation will be studied using flow cytometry: CD11b, CD11c, CD13, CD16, CD32, CD35, CD38, CD62L, CD64, CD66b, CD88, CD284, HLA-ABC and HLA-DR.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to 80 Years

Healthy volunteers

Inclusion criteria

* neuroinfections or acute intestinal infections; * the possibility of blood sampling within a period of up to 24 hours from the moment of admission to hospital; * less than 72 hours passed after the onset of the disease and until blood was collected;

Exclusion criteria

* refuse of patient to participate in the trial; * more than 24 hours passed after the patient entered the hospital; * the disease began more than 72 hours ago; * concomitant severe acute and chronic diseases; * malignant neoplasms, treatment at a time of less than 1 year, autoimmune diseases in the phase of exacerbation; * pregnancy/lactation; * HIV, Hepatites B/C; * active tuberculosis; * alcohol use disorder/drug addiction.

Design outcomes

Primary

Measure	Time frame	Description
Sensitivity of the diagnosis of bacterial vs viral acute infection	1 month	Sensitivity of the diagnosis of bacterial vs viral acute infection using proposed method
Specificity of the diagnosis of bacterial vs viral acute infection	1 month	Specificity of the diagnosis of bacterial vs viral acute infection using proposed method

Outcome results