Bacteremia, Infective Endocarditis
Conditions
Keywords
Diagnosis, Blood Culture
Brief summary
To evaluate the performance of a single high volume blood culture sampling strategy versus the actually used multiple sampling strategy for the diagnosis and categorization of infective endocarditis according to the Duke-Li classification in a Population of adults suspected of infective endocarditis.
Interventions
PROCEDUREBlood Culture
For each patient, one single high volume blood culture (3 aerobic and 3 anaerobic of 8 to 10 mL each, numbered), and then 2 samples of 16 to 20 mL (one aerobic bottle and one anaerobic for each sample).
Sponsors
Central Hospital, Nancy, France
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients older than 18 years * Suspected endocarditis: Patients with Duke-Li-ESC 2015 classification as a major morphological criterion or at least two minor criteria, other than a microbiological criterion, will be considered suspicious of infectious endocarditis. * Not objecting to their inclusion in the study after delivery and explanation of the information form. * Absence of microbiological documentation sought or available at the time of inclusion (a patient having already had negative blood cultures or being identified during the screening can be included).
Exclusion criteria
* Antibiotherapy adapted to a situation of endocarditis, introduced more than 24 hours or stopped for less than 7 days in case of therapeutic window. * Any previous antibiotic therapy in the 7 days preceding the inclusion leading to an improvement in the clinical symptomatology. * State of consciousness not allowing loyal information.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Compared performance of a single high volume blood culture vs. multiple blood culture of usual volume for the diagnosis of infective endocarditis | At the end of the hospitalization, or at the time of death if occurred during hospitalization; i.e. an average of 6 weeks after the inclusion | Sensitivity/Specificity/Accuracy of positive bloodculture sets vs. final diagnosis assessed by the medical team (gold standard) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Diagnostic performance of the single high volume blood culture for the diagnosis of infective endocarditis | At the end of the hospitalization, or at the time of death if occurred during hospitalization; i.e. an average of 6 weeks after the inclusion | according to the level of suspicion of infective endocarditis, the type of microorganism involved, the underlying cardiopathy. |
| Measuring the nursing time required for both sampling methods. | At T0, i.e. at the inclusion of the patient | Time for blood culture setting, processing and sending |
| Diagnosis of infective endocarditis: confirmed, possible or excluded | At the end of the hospitalization, or at the time of death if occurred during hospitalization; i.e. an average of 6 weeks after the inclusion | Applying the Classification of Duke-Li according to the modified diagnostic criteria of the European Society of Cardiology Recommendations 2015. |
Countries
France
Outcome results
None listed