Nasal Polyps
Conditions
Keywords
nasal epithelium stem cells, Hippo-YAP pathway, proliferation, differentiation
Brief summary
Hippo-YAP pathway is a newly discovered pathway controlling the growth, and plays important roles in the regulation of stem cell function and tissue regeneration. Previously we found that a higher expression of YAP in NP, and LPS was able to activate the hippo-YAP pathway in NESCs. Thus, we hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway.
Detailed description
Studies have shown that the development of nasal polyps (NP) are closely related to the epithelial remodeling.The investigators have shown that nasal epithelial stem cells(NESCs) derived from NP had reduced growth and proliferation dynamics in vitro culture,and differentiated into ciliated cells with impaired cilia architecture and ciliogenesis.Further studies demonstrated that NESCs were involved in the aberrant damage repair process of the epithelial remodeling in NP.Hippo-YAP pathway is a newly discovered pathway controlling the growth,and plays important roles in the regulation of stem cell function and tissue regeneration.Previously the investigators found that a higher expression of YAP in NP,and LPS was able to activate the hippo-YAP pathway in NESCs.Thus,the investigators hypothesize that the aberrant proliferation and differentiation of NESCs could be regulated via the hippo-YAP pathway,which may result in the epithelial remodeling.In this study,the investigators intend to investigate the mechanism of the hippo-YAP pathway in regulating aberrant proliferation and differentiation of NESCs and explore its upstream signaling and downstream target genes,using the NESCs gas-liquid interface culture system,and a silencing and overexpression of the hippo-YAP pathway component strategy.The investigators aim to unravel the effect and mechanism of the hippo-YAP pathway in the epithelial remodeling of NP,providing new targets and strategy for the improvement or reversal of NP epithelial remodeling.
Interventions
PROCEDUREfunctional endonasal sinus surgery
collect nasal polyps from patients
PROCEDUREnasal septal construction
collect inferior turbinates from patients as control arm
Sponsors
National University of Singapore
Third Affiliated Hospital, Sun Yat-Sen University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Clinical diagnosis of nasal polyps or deviation of nasal septum based on CT * Must be able to withstand surgery * At least 18 years old * A sufficient level of education to understand study procedures and be able to communicate with site personnel and adhere to the follow-up; * Accepted informed consent verbally and in writing
Exclusion criteria
* The Poor Health * Be allergic to anesthetics
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| location of YAP in tissues | through study completion, an average of 2 years | through Immunohistochemistry |
| protein expression level of YAP in tissues | through study completion, an average of 2 years | through western blot |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| activation of hippo-YAP in NSECs on protein aspect | through study completion, an average of 2 years | Western Blot |
| expression level of Th1 cytokines in the tissues | through study completion, an average of 2 years | through Flow cytometry |
| activation of hippo-YAP in NSECs on gene aspect | through study completion, an average of 2 years | quantitive real time PCR |
| activation of hippo-YAP in NSECs | through study completion, an average of 2 years | Flow cytometry |
| expression level of Th17 cytokines in the tissues | through study completion, an average of 2 years | through Flow cytometry |
Countries
China
Contacts
Primary Contacthuiyi DENG, MD
Backup Contactqintai YANG, PhD
Outcome results
None listed