Protein Metabolism
Conditions
Keywords
Endurance exercise, Dietary protein
Brief summary
A randomized interventions study using a crossover design. Ten well trained triathletes or bikeriders will be included for to undergo two trail separated by about 3 weeks. The night before the trail each subject will performe a high intense interval trainings session on an ergometer bike. during the night an infusion of aminoacid tracers will be initiated. The subject is woken at 6.30 a.m. Blood samples and a musclebiopsy will be collected prior to the morning bike ride at 8.00a.m. Immediately before the morning bike ride is started the subject will ingest a drink of 0,5g protein or a non-caloric placebo (random order). During the bike ride and the following 4,5 hours blood will be drawn with regular intervals. at time point 0, 60 and 180 min after the training session biopsies are collected.
Interventions
OTHERProtein ingestion
A protein drink was ingested prior to the 90min bike ride in one of the completed trails (order randomised).
OTHERNon-caloric placebo ingestion
A placebo drink was ingested prior to the 90min bike ride in one of the completed trails (order randomised).
Sponsors
Arla Foods
Team Denmark
Innovation Fund Denmark
University of Aarhus
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
HEALTH_SERVICES_RESEARCH
Masking
SINGLE (Subject)
Masking description
Masking was done by providing similar tasting drinks at both trails. One was placebo, one was protein a drink
Eligibility
Sex/Gender
MALE
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy well-trained triathletes or cyclists * VO2max \> 55 ml O2/kg/min * Training and has been training more than 7 times a week the last 6 months.
Exclusion criteria
* VO2max \< 55 ml O2/kg/min * Has chronic disease * has incjected with tracer on a previous occasion.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Muscle protein fractional synthetic rate (FSR) | FSR was measure over at time period of six hours |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Tracer kinetics dilution model | six hours | A-V tracer dilution model was applied across the forearm. |
| Muscle signalling | Determined in biopsies sampled before and immediatly after 90min exercise in addition to one and four hours after exercise. | Muscle signalling will be determined using western blotting in each muscle biopsy |
| Nitrogen excretion | Urine is collected during two periods. Collection period one: 12 hours prior to morning exercise. Collection period 2: During and four hours following exercise. Blood samples is drawn repeatedly from before to four hours after exercise. | nitrogen excretion is determined by Plasma and urinary carbide analysis as well as plasma NH4+. |
Outcome results
None listed