Infertility, Male
Conditions
Brief summary
In the current era of assisted reproductive techniques where technology can help overcome defects in sperm function, the value of semen analysis has become even more inaccurate. Initial reports of intracytoplasmic sperm injection revealed its ability to bypass the natural selection process and enable men with severely impaired semen parameters to achieve both clinical pregnancy and live birth
Interventions
DIAGNOSTIC_TESTSperm DNA integrity by flow cytometry
flowcytometric test where sperm DNA breaks are evaluated indirectly through the DNA denaturability. The assay measures the susceptibility of sperm DNA to acid-induced DNA denaturation in situ, followed by staining with the fluorescence dye acridine orange. By using a flow cytometer 5.000-10.000 sperm can be analyzed within few seconds and thus provide a less subjective result compared to the World health organization analysis where only 1-300 cells are analyzed.
Sponsors
Assiut University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
MALE
Age
20 Years to 50 Years
Healthy volunteers
No
Inclusion criteria
1. Infertile male patients with abnormal semen parameters. 2. Normal female partner aged \<38 years.
Exclusion criteria
1. Female patients with obvious infertility factor. 2. Male patients with azoospermia. 3. Male patients with systemic diseases. 4. Male patients receiving cytotoxic therapy. 5. Male patients with genital pathology as cryptorchidism.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| clinical pregnancy rate | 4 weeks |
Countries
Egypt
Outcome results
None listed