Metabolism Disorder, Mitochondrial Alteration, Obesity, Physical Activity
Conditions
Brief summary
Considering that the failure of the treatment of obesity is justified by the multifactorial pathophysiology of this morbidity, the present project has the following hypotheses: 1. The occurrence of obesity is due to the derange,ent of mitochondrial energy metabolism ; 2. The unbalance is therapeutically modified through physical training ; 3. Obesity courses with the break-down in energy metabolism mitochondrial disease associated with systemic inflammatory characteristics that can be corrected through a combined long-term physical training program. This study have as objective : to analyse changes in mitochondrial function, inflammatory profile, oxidative stress and energy metabolism caused by concurrent physical training in obese women.
Detailed description
Specific objectives: Body composition by deuterium oxide; Metabolic rate of resting and oxidation of substrates by indirect calorimetry; Proinflammatory cytokines Anti-inflammatory cytokines Oxidative Stress: Malondialdehyde, Superoxide Dismutase, Glutathione-Peroxidase; Fatty acids: ceramide and palmitate; Mitochondrial respiration and citrate synthase enzyme; Quantify and qualify: mitochondrial number, endoplasmic reticulum structure, adipose cell size; Gene expression, quantify by microscopy and analyze the protein by western blot. The study began with 20 women, however, there was withdrawal of 6, ending with 14 women.
Interventions
OTHERPhysical Training
Intervention with concurrent physical training: strength and aerobic exercises in the same session. Duration: 2 weeks of adaptation to physical exercise, 8 weeks of training. Frequency: 3 times a week. Time: 55 minutes each session. Intensity: 75 to 90% of maximum heart rate.
Sponsors
University of Sao Paulo
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 40 Years
Healthy volunteers
No
Inclusion criteria
* This study included women with obesity (BMI of 30 to 40 kg / m²), sedentary, with no associated comorbidity, convenience sample
Exclusion criteria
* Women who have undergone bariatric surgery, menopause, cancer or any metabolic disease, smokers, alcoholics, who are in use of drugs that act directly on the metabolism and that have medical impediment to the practice of physical exercise.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes Body weight | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | Body weight was measured by digital balance before and after the intervention |
| Changes Body composition | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | The change in body composition through deuterium oxide was evaluated. |
| Changes White adipose tissue biopsy | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | A subcutaneous tissue sample was collected for analysis of: mitochondrial respiration, citrate synthase enzyme, gene expression (UCP1, 2 and 3). |
| Changes Indirect calorimetry | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | With a gas analyzer (indirect calorimeter), we evaluated the metabolic rate and rest (REE) and oxidation of substrates (Lipids and carbohydrates). |
| Changes in fatty acids | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | Collected in lithium heparin tubes, they were centrifuged. |
| Changes oxidative stress | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | Collected in lithium heparin tubes, they were centrifuged. |
| Changes inflammatory cytokines | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | Collected in lithium heparin tubes, they were centrifuged. |
| Changes in total cholesterol | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | Collected in lithium heparin tubes, they were centrifuged. |
| Changes Physical Performance | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | Based on the Shuttle Walking Test adaptation. |
| Changes in Determination of Lactate | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | Blood samples were collected by manual puncture of the earlobe in previously calibrated and heparinized capillary tubes, stored in eppendorf with sodium fluoride. Analyzed by electrochemical lactate analyser. |
| Changes Food intake | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | Food registry of 3 days, the quantification of the daily intake of nutrients will still be made using software. |
| Changes Nitrogen Balance | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | Through the collection of urine of 24 hours the dosage of urinary nitrogen will be made by the chemiluminescence method for determination of protein nitrogen. |
| Changes Telomere length | Two times: (1) First day and (2) 10 weeks after adaptation and intervention | peripheral blood in ethylenediaminetetraacetic acid tubes and genomic DNA was automatically extracted from Peripheral Blood Mononuclear Cell. The relative quantification of Telomere length was determined using the telomere to single copy gene ratio by Quantitative Polymerase Chain Reaction (qPCR). |
Countries
Brazil
Outcome results
None listed