Human Brown Adipose Tissue and Mitochondrial Respiration

Human Brown Adipose Tissue Histological and Mitochondrial Respiratory Analysis

Status

UNKNOWN

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT03111719

Enrollment

180

Registered

2017-04-13

Start date

2010-01-04

Completion date

2021-10-10

Last updated

2020-02-21

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Adipose Tissue, Brown, Aging, Mitochondrial Metabolism Disorders, Obesity

Brief summary

This study is focused on measuring mitochondrial activity of human brown adipose tissue. During surgery, adipose tissue biopsies from white and brown adipose tissue will be collected from the deep neck area. These biopsies will be used to examine mitochondrial function via histological analysis, biochemical analysis and in vitro experiments based on precursor cells present in the biopsies.

Interventions

None listed

Study design

Observational model

COHORT

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

ALL

Age

18 Years to 65 Years

Healthy volunteers

Inclusion criteria

* Indication for surgery in the supraclavicular and neck region (more specific patients diagnosed with hyperparathyroidism, struma, benign thyroid gland tumours, implantation of a vagal neurostimulator for epilepsia and cervical neurologic disorders) * In the thyroid gland group, euthyroid function

Exclusion criteria

* Pre-operatively diagnosed malign tumour * Patients undergoing acute surgery because of critical illness

Design outcomes

Primary

Measure	Time frame	Description
Mitochondrial respiration in human brown	8 weeks	Measure of mitochondrial respiration in human brown adipose tissue biopsies and measure mitochondrial respiration in cells derived from human brown adipose tissue biopsies. With high resolution respirometry , oxygen consumption in mitochondria will be determined using different substrates that stimulate mitochondrial activity including norepinephrine which is a natural agonist to increase mitochondrial activity in brown adipose tissue. During these experiments mitochondrial complex activity can be inhibited using specific inhibitors.

Secondary

Measure	Time frame	Description
Characterise human brown adipose tissue	8 weeks	Perform histological analysis and biochemical analysis in brown adipose tissue biopsies and compare to skeletal muscle. Via qPCR analysis, western blotting and immunfluorescence we will examine UCP1 expression in BAT.

Countries

Netherlands

Contacts

Primary ContactWouter D Van Marken Lichtenbelt, Professor

markenlichtenbelt@maastrichtuniversity.nl+31(0)433881629

Outcome results