Cognitive Function, Healthy
Conditions
Brief summary
Impaired metabolism and the gene apolipoprotein E (ApoE) are independent risk factors for cognitive impairment and dementia. In humans, there are three major versions of apoE (E): E2, E3, and E4. Some studies suggest that the different versions of apoE have varying effects on whole body and brain metabolism. The goal of this project is to better understand the relationship between apoE and metabolism. This will help investigators identify new targets for the prevention and treatment of cognitive decline and dementia.
Detailed description
The investigator hopes determine whether a participant's version of ApoE affects their metabolic rate at rest and during a cognitive challenge. To determine this, the investigator will measure basal (resting) metabolic rates using a technique called indirect calorimetry (IC). This is accomplished by wearing a loose-fitting face mask that measures oxygen intake and carbon dioxide output. To more accurately measure the amount of carbohydrates, fats and proteins that the body is metabolizing, participants will be asked to provide two urine samples (one at the beginning, and one at the end, of the study). To determine what version of ApoE the participant inherited, the investigator will conduct a genetic test on DNA isolated from the participant's blood.
Interventions
OTHERResting State Indirect Calorimetry
The investigator will measure the amount of oxygen participants breathe in and the amount of carbon dioxide they breathe out using a portable unit. Participants will be asked wearing a loose-fitting face mask that measures oxygen intake and carbon dioxide output.
GENETICApoE
The investigator will conduct a genetic test on DNA isolated from from the participants blood. Participants will be asked to provide about 2 tablespoons.
DIAGNOSTIC_TESTMetabolism
To more accurately measure the amount of carbohydrates, fats and proteins that the participants bodies metabolize, participants will be asked to provide two urine samples (one at the beginning, and one at the end, of the study). The total amount of urine to be collected is approximately 4 teaspoons.
OTHERCognitive Challenge
Participants will be asked to follow simple instructions that are displayed on computer screen.
Sponsors
Lance Johnson
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Inclusion criteria
* all races/ethnicities * ages 18-65 * cognitively normal * good health
Exclusion criteria
* pregnant or breastfeeding. * have a bleeding disorder, * have a history of stroke, seizures, Parkinson's disease, history of head injury with loss of consciousness, or other dementing disorder. * have a history of alcoholism or drug abuse * have a history of schizophrenia or currently suffer from bipolar disorder or major depression. * have vision or hearing loss severe enough to interfere with cognitive testing
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Indirect Calorimetry | 4 hours | Measure resting state respiratory quotient in cognitively normal participants with various ApoE genotypes |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Respiratory Quotient (RQ) | 4 hours | Measure RQ during a cognitive challenge in cognitively normal individuals with various ApoE genotypes |
Other
| Measure | Time frame | Description |
|---|---|---|
| Urea Nitrogen Output | 4 hours | Improve RQ measure accuracy by determining urinary urea nitrogen output in collected urine samples from these subjects. |
| Genotype | 4 hours | Genotype subjects using DNA isolated from collected blood samples. |
Countries
United States
Outcome results
None listed