Mesenchymal Stem Cells
Conditions
Keywords
stem cell, liposuction, regenerative medicine
Brief summary
The aim of the study is to evaluate a physiological concentration of adipose tissue-derived stem cells (ASC) in excised fat versus fat harvested by liposuction. Participants will act as their own control as both extraction methods are performed on each participant.
Detailed description
Adipose tissue-derived stem cells are of great interest due to their relevance in regenerative medicine and tissue engineering. A reason, being their capacity for multipotent differentiation, tissue repair and immune modulation. To evaluate a physiological concentration level of ASCs in human fat we investigate two extraction methods of fat, excision and liposuction. This is done through a standardised setup with patients acting their own control. The study is carried out in accordance with the guidelines provided by the International Federation for Adipose Therapeutics and Science and International Society for Cellular Therapy.
Interventions
PROCEDURELiposuction
Fat harvest by liposuction (Colemans method)
Sponsors
University of Aarhus
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Participants will act as their own control. Each participant will receive both excision and liposuction of fat.
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Eligible women - over the age of 18 * Deemed suitable for abdominoplasty with abdomen pendens, fleur de lis, panniculectomy or belt lipectomy. * Able to read and understand Danish
Exclusion criteria
* Current smokers - patients not having paused for a minimum of four weeks prior to surgery. * Prior or current adjuvant chemo therapy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Stem cell yield | At baseline right after isolation. | Stem cell yield measured by antibody stained flow cytometry. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Growth kinetics | Cultured for 7 days and counted. | Growth kinetics measured by culturing. The population doubling time is determined using log phase of growth |
| Adipogenic, chondrogenic and osteogenic differentiation. | Through study completion, an average of 1 year. | Differentiation assays for adipogenic, chondrogenic and osteogenic differentiation with Oil Red O staining, Safranin O staining and Alizarin red S staining. |
Outcome results
None listed