Choledocholithiasis, Cholelithiasis
Conditions
Brief summary
Bile duct stones is a common biliary tract disease, which is characterized by high morbidity and frequent recurrence. Endoscopic retrograde cholangiopancreatography (ERCP) is an effective therapy for common bile duct stones, and endoscopic sphincterotomy (EST) which associated with recurrent cholangiolithiasis often carried out on difficult intubation or extracting stones, probably due to enhanced reflux of intestinal contents that changes the microenvironment. Patients with cholangiolithiasis were consecutively recruited and their bile was collected intra-operatively for high-throughput experiments. Pyrosequencing of 16S ribosomal RNA gene was performed to characterize the microbiota in the bile and other body fluids. A liquid chromatography mass spectrometry-based method was used to profile bile composition. Clinical manifestation, microbiome, and bile composition were compared between patients with or without recurrent of bile duct stones. The aim of our study was to identify the impact of microbiomes on the recurrent of bile duct stones after ERCP+EST therapy.
Interventions
None listed
Sponsors
The Second Hospital of Nanjing Medical University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
No
Inclusion criteria
1. Patients with recurrent common bile duct stones. 2. No antibiotics or probiotics are used for nearly three months. 3. In addition to common bile duct stones, possibly without other digestive diseases.
Exclusion criteria
1. Significant neurological or psychiatric disorders (psychotic disorders, dementia or seizures) that would prohibit the understanding and giving of informed consent. 2. Pregnant or lactating females. 3. Active Hepatitis B or C or history of an HIV infection. 4. Active uncontrolled infection. 5. Billroth II or roux-en Y gastrointestinal alteration people.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes of Bacteria in Body Fluid | 3 years | measure the changes of Bacteria in Body Fluid |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Stone diameter | 3 years | measure the stone diameter |
| EST size | 3 years | measure the EST size |
| Stone type | 3 years | test the stone type |
| Bile acid composition(Mass spectrometry or Chromatography) | 3 years | measure the bile acid composition with Mass spectrometry or Chromatography |
Countries
China
Contacts
Primary ContactMiao Lin
Outcome results
None listed