Gastric Cancer, Polymerase Chain Reaction
Conditions
Keywords
Polymerase Chain Reaction, gastric cancer, citology, peritoneal washing, CK20, CEA, BER-EP4
Brief summary
Gastric carcinoma is a disease with a high mortality. Peritoneal washing cytology has a recognized prognostic utility. However sensibility of this study is not appropriate. Studies with PCR (polymerase chain retrotranscription) can increase sensibility and accurate staging information. Objective: To analyze the sensibility and specificity of the expression of CK20, CEA and Ber-EP4 with PCR technique in peritoneal washings of advanced gastric carcinoma without clinical evidence of peritoneal carcinomatosis. Methodology: Retrotranscription analysis (RT-PCR) and quantitative PCR (qPCR) of CK20, CEA and Ber-EP4 in peritoneal washing. Test the feasibility of qPCR with this new marker (Ber-EP4). Comparison of the results with data obtained with conventional cytology. Study of peritoneal recurrence.
Interventions
None listed
Sponsors
Corporacion Parc Tauli
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patients with resectable advanced gastric cancer * Informed consent
Exclusion criteria
* Macroscopic peritoneal carcinomatosis * Non operable patients
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| specific markers in peritoneal cells | during surgical intervention | Detection of CK20, CEA and BER-EP4 by PCR technique in peritoneal washing of advanced gastric cancer |
Countries
Spain
Contacts
Primary ContactAlexis Luna, MD PhD
Outcome results
None listed