HIV-1-infection
Conditions
Keywords
HIV-1, Colo-rectal transmission, seminal plasma
Brief summary
The objective of our project is to determine the infected seminal cell types and the molecular mechanisms involved in HIV cell-associated transmission in the colo-rectal mucosa, using conditions as close as possible to real life, i.e. seminal leukocytes and seminal plasma from HIV-infected donors and tissue explants
Detailed description
This project relies on: * our expertise of HIV in semen and male genital tract and our experience in organotypic cultures; * a network of infectious diseases and reproductive biology clinicians to collect, analyze and process seminal cells and seminal plasma from HIV+ donors; * the expertise in confocal microscopy and ex vivo colo-rectal model. State of the art culture of colo-rectal tissues, primary cell culture and infection, confocal microscopy, flow cytometry, Polymerase Chain Reaction and Luminex assays for cytokine measurements will be the methodologies primarily used in this project.
Interventions
OTHERblood sample
A sample of HIV-1 infected patients's blood is collected
OTHERsperm sample
A sample of HIV-1 infected patients's sperm is collected
Sponsors
ANRS, Emerging Infectious Diseases
Institut National de la Santé Et de la Recherche Médicale, France
University Hospital, Toulouse
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
18 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
* HIV-1 infected patients who have never been treated by antiretroviral therapy * Signature of informed consent * Affiliation to social service
Exclusion criteria
* Patients' general condition not allowing protocol follow-up * Patients with an ejaculation disorder * Patients unable to perform semen sampling * Patients with chemotherapy or radiotherapy antecedents * Patients with very severe oligospermia (≤1million/mL of spermatozoa) * Patients with active genital infection * Protected subjects
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Determine the efficiency of seminal cell associated infection. | 1 years | The efficiency of seminal cell associated infection will be determinated in the colo-rectal explants. |
| Evaluate the potential of seminal HIV infected cells migration and adhesion. | 1 years | Assess the potential of seminal macrophages and CD4 T cells to adhere and transmigrate. |
| Characterize seminal HIV infected cells. | 1 years | Macrophages and CD4+ T lymphocytes characterization. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Identify molecules expressed and involved in seminal cell adhesion and transmigration. | 1 years | Identify key adhesion molecules expressed by seminal leucocytes and epithelial cells involved in seminal cell adhesion/transmigration. |
| Identify seminal and mucosal molecules mediating seminal cell translocation. | 1 years | Seminal and mucosal soluble molecules mediating seminal cell translocation will be characterized (passive diffusion or active transmigration). |
| Determine the impact of seminal plasma/cells exposure on mucosal immune cell. | 1 years | Mucosal immune cell activation, trafficking and the factors involved, will be evaluated. |
Countries
France
Outcome results
None listed