University of Wisconsin Solution Versus New Parathyroid Transport Solution for Parathyroid Allotransplantation

University of Wisconsin Solution Versus New Parathyroid Transport Solution for Parathyroid Allotransplantation: Effects on Calcium Sensing and Vitamin D Receptor During Cold Ischemia

Status

UNKNOWN

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT03051308

Enrollment

Registered

2017-02-13

Start date

2017-03-01

Completion date

2017-07-01

Last updated

2017-02-13

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Parathyroid Allotransplantation

Keywords

Parathyroid cell viability, Ischemia, Parathyroid allotransplantation, Vitamin D Receptor, Calcium Sensing Receptor

Brief summary

Compare the functionality of new parathyroid transport solution (NPTS) with University of Wisconsin Solution (UW) on parathyroid glands. Also assess its effects on cell viability, parathormone (PTH) secretion, calcium-sensing receptor (CaSR) and vitamin D receptor (VDR) levels during cold ischemia.

Detailed description

Parathormone (PTH) and vitamin D are two critical hormonal regulators of calcium homeostasis. There is an important relationship between the PTH release and vitamin D receptor for controlling hormonal systems. Also calcium-sensing receptor plays a crucial role in regulating parathormone secretion which means controlling the calcium-dependent systemic ion homeostasis. Cold ischemia protects organs and tissues by slowing their metabolism. The cold ischemia of the transplant tissue is a very important process. The University of Wisconsin (UW) solution substantially improves graft preservation and consequently increases patient survival. The researchers newly developed NPTS for transportation of parathyroid glands. For the comparision the researchers measured the parathyroid cell viability and the calcium sensing and vitamin D receptor density of the parathyroid cells at the 0, 6, 12 and 24 hours of cold ischemia.

Interventions

COMBINATION_PRODUCTNPT and UW Solution

Tissue preservation solutions for transplantation

Study design

Allocation

RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

18 Years to 80 Years

Healthy volunteers

Inclusion criteria

* Patients who have parathyroid hyperplasia due to chronic renal failure.

Exclusion criteria

* Patients who refused to join the study * Patients younger than 18 years and older than 80 years. * Patients who have hepatitis B, * Patients who have hepatitis C, * Patients who have human immunodeficiency virus (HIV).

Design outcomes

Primary

Measure	Time frame	Description
Cell viability of Parathyroid cells after isolation process	0 hour	After removal of hyperplastic parathyroid glands, each gland will keep in ice cold NPTS and UW solution for transportation to laboratory. Then the investigators will measure the viability of Parathyroid cells after isolation process.
Cell viability of Parathyroid cells after 6 hour of cold ischemia	6 hour	After removal of hyperplastic parathyroid glands, each gland will keep in ice cold NPTS and UW solution for transportation to laboratory. After 6 hours the investigators will measure the viability of Parathyroid cells after isolation process.
Cell viability of Parathyroid cells after 12 hour of cold ischemia	12 hour	After removal of hyperplastic parathyroid glands, each gland will keep in ice cold NPTS and UW solution for transportation to laboratory. After 12 hours the investigators will measure the viability of Parathyroid cells after isolation process.
Cell viability of Parathyroid cells after 18 hour of cold ischemia	18 hour	After removal of hyperplastic parathyroid glands, each gland will keep in ice cold NPTS and UW solution for transportation to laboratory. After 18 hours the investigators will measure the viability of Parathyroid cells after isolation process.
Cell viability of Parathyroid cells after 24 hour of cold ischemia	24 hour	After removal of hyperplastic parathyroid glands, each gland will keep in ice cold NPTS and UW solution for transportation to laboratory. After 24 hours the investigators will measure the viability of Parathyroid cells after isolation process.

Secondary

Measure	Time frame	Description
Parathormone Release of Parathyroid Cells	1 day	The investigators will measure the parathormone level from cultured parathyroid cells

Countries

Turkey (Türkiye)

Contacts

Primary ContactErhan Aysan, Prof. M.D.

erhanaysan@hotmail.com+902124531700

Backup ContactBeyza Goncu, Msc

bsgoncu@gmail.com+902124531700

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026