Atherosclerosis, Platelet Aggregation, Spontaneous, Surgery
Conditions
Keywords
Thrombocyte aggregation, Fish oil, Alternative medicine, Point of care testing, Coagulation monitoring
Brief summary
Many patients undergoing surgery use naturopathic drugs, including fish oil. Fish oil has been reported to increase bleeding in patients through inhibiting platelet aggregation and prolonging of clot formation time. The Swedish Medical Products Agency recommends that patients stop taking the naturopathic medicines including fish oil two weeks prior planed surgery. The aim of this study is to examine the effects of fish oil given to healthy volunteers using point of care coagulation assessment and flow dependent Cellix instrument before and after intake of fish oil.
Detailed description
Healthy volunteers will be given maximum dose of fish oil for 7 days. Blood samples will be collected before and immediately after the 7 days of intake and analysed using point of care coagulation assessment instruments and flow dependent platelet aggregation using a microfluid flow controlled system (Cellix). For point of care coagulation assessment the multiple electrode platelet aggregometer Multiplate, the viscoelastic rotational thromboelastometers ROTEM and Sonoclot will be used. Flow cytometry may also be utilized.
Interventions
DRUGFish oil
Blood samples before and after 10 day intake of maximum dose fish oil
Sponsors
Region Skane
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Before and after
Eligibility
Sex/Gender
ALL
Age
19 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Non smoking healthy volunteers with written consent to participate
Exclusion criteria
* Medication affecting coagulation or platelets within 14 days priori to inclusion
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Thrombocyte aggregation measured with multiple electrode aggregometry | Change of thrombocyte aggregation directly after intake period of 7 days compared to baseline | Multiple electrode aggregometry gives a area under the curve proportional to thrombocyte aggregation |
| Thrombocyte aggregation measured with Cellix's microfluidic pumps and biochips | Change of thrombocyte aggregation directly after intake period of 7 days compared to baseline | Cellix's microfluidic pumps and biochips gives an thrombosis area proportional to thrombocyte aggregation |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Coagulation measured with the viscohemostatic instrument ROTEM | Change of coagulation directly after intake period of 7 days compared to baseline | Viscohemostatic instrument |
| Coagulation measured with the viscohemostatic instrument and Sonoclot | Change of coagulation directly after intake period of 7 days compared to baseline | Viscohemostatic instrument |
Countries
Sweden
Outcome results
None listed