Bariatric Surgery, Obesity, Morbid
Conditions
Keywords
Gastric sleeve, Gastric bypass
Brief summary
After gastric sleeve patients undergo a substantial weight loss, but the physiological mechanisms behind the weight loss is not fully elucidated. Studies suggest that gut-hormone secretion is altered comparable to what is also seen after Roux-en-Y gastric bypass, however to which extent is not fully established and furthermore, the mechanisms behind an altered secretion are unclear. The purpose of this study is to investigate absorption and postprandial metabolism of glucose and protein in addition to gut hormonal responses after gastric sleeve compared with a group of un-operated subjects machted on sex, age and BMI. Furter, a group of Roux-en-Y gastric bypass operated patients matched on pre-operative BMI, current BMI, sex and age will be included for comparison.
Interventions
DIAGNOSTIC_TESTMixed meal test with double tracer technique
Sponsors
University of Copenhagen
Hvidovre University Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
25 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
SG ang RYGB group: Inclusion Criteria: * Uncomplicated RYGB or SG \> 12 month prior to inclusion * Weight stable (+/- 3 month the last month) * Written informed consent
Exclusion criteria
* Diabetes (HbA1c\>48 or Fasting plasma-glucose\>6.1 mM) * Pregnancy and/or breastfeeding * Inadequately treated thyroid disease * Serious heart or respiratory illness * Haemoglobin \< 6,5 mM. Un-operated control group: Inclusion Criteria: * Written informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Glucose absorption | 0 - 360 minutes | Between groups difference in peak Rate of appearance (Ra) of oral glucose tracer SG vs. CON |
| Protein absorption | 0 - 360 minutes | Between groups difference in peak Ra of oral aminoacid tracer SG vs. CON |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Glucose kinetics | 0 - 360 minutes | Between groups differences in AUC Ra of oral glucose tracer |
| Protein kinetics | 0 - 360 minutes | Between groups differences in AUC Ra of oral aminoacid tracer |
| Insulin secretion | 0 - 240 minutes | Between groups differences in iAUC of serum insulin and C-peptide |
| Glucagon-like Peptide 1 (GLP-1) secretion | 0 - 240 minutes | Between groups differences in iAUC of plasma concentration of GLP-1 |
| Glucose-dependent Insulinotropic Peptide (GIP) | 0 - 240 minutes | Between groups differences in iAUC of plasma concentration of GIP |
| Glucose absorption | 0 - 360 minutes | Between group difference in peak Ra of oral glucose comparing SG vs. RYGB |
| Glucagon secretion | 0-240 minutes | Between groups differences in iAUC of plasma cncentration of glucagon |
| Peptide YY | 0-240 minutes | Between groups differences in iAUC of plasma concentration of PYY |
| Endogenous glucose production | -120 - 360 minutes | Calculated via double tracer tecnique both in the fasting and post prandial state. |
| Lipolysis | -120 - 360 | Between groups differences in Rate of Disappearance of glycerol tracer. |
| Ghrelin secretion | 0 - 240 minutes | Between groups differences in iAUC of plasma concentration of ghrelin |
| Protein absorption | 0 - 360 minutes | Between groups differences in peak Ra of oral aminoacid tracer comparing SG vs. RYGB |
Countries
Denmark
Outcome results
None listed