Infertility, Female
Conditions
Keywords
Endometrium, Cytokines, Endometrial secretion, Implantation, Endometrial scratching
Brief summary
This study investigates the impact of endometrial scratching on endometrial secretion, when correlated to pregnancy.
Interventions
OTHERPipelle de Cornier
RCT with endometrial scratching prior to hormone treatment in ART. Aspiration of uterine secretion at transfer day
Sponsors
University of Aarhus
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
DIAGNOSTIC
Masking
NONE
Intervention model description
Randomized clinical trial
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
No
Inclusion criteria
* IVF or ICSI patients with ≥1 previous implantation failure, despite transfer of a top quality embryo or blastocyst. * Antagonist treatment * FSH: 2-12 IU/L * BMI: 18-32 * Regular menstrual cycle * Written consent
Exclusion criteria
* Suspected intrauterine abnormalities Planned use of specialized media or AHA
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Cytokine profile of the uterine secretome after therapeutic endometrial scratching | Within 12 months | The secretome is analyzed using a Multiplex analysis with 45 cytokines, chemokines and growth factors from ProcartaPlex Immunoassay Kit, Affymetrix, eBioscience. The samples are normalized for total protein content using a Nanodrop spectrophometer, and the protein composition is further determined by sodium dodecyl sulphate-polyacrylamide gel electropheresis (SDS-PAGE). |
| Immunoassay Kit: | Within 12 months | BDNF, EGF, Eotaxin, FGF-2, GMCSF, GRO-alpha, HGF, IFN alpha, IFN gamma, IL-1RA, IL-1alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL17-A, IL-18, IL-21, IL-22, IL-23, IL-27, IL-31, IP-10, LIF, MCP-1, MIP-1 alpha, MIP-1 beta, NGF beta, RANTES, PDGF-BB, PÅIGF-1, SCF, SDF-1 alpha, TNF alpha, TNF beta, VEGF-A, VEGF-D |
Countries
Denmark
Outcome results
None listed