Severe Sepsis
Conditions
Keywords
Platelet, severe sepsis, inflammation, S. aureus, biomarkers
Brief summary
Sepsis represents a serious public health issue characterized by a complex inflammatory response. In addition to their hemostatic role, platelets display inflammatory functions by secreting a variety of immunomodulatory factors and interacting with circulating immune cells. The investigators postulate that, in severe sepsis, platelets become activated and release amounts of different soluble inflammatory molecules that contribute to sepsis-associated inflammation. First, the investigators propose to assess whether severe sepsis impairs the ability of platelets to release soluble CD40L (sCD40L), an powerful platelet-derived immunomodulatory molecule, in ICU patients with S. aureus documented infection, ICU patients with documented infection involving other bacterial species, compared to ICU patients with inflammation of noninfectious origin and healthy blood donors. Then, the investigators wish to assess whether the bacterial species affects the release of platelet sCD40L and by an extensive screening of platelet soluble factors, the investigators propose to set up profiles of inflammatory molecules associated with the type of infection. Finally, the investigators will analyze platelets' activation state and their association with circulating immune, according to the type of infection. Therefore, this project is expected to assess to which extent the platelet inflammatory function is super-activated in severe sepsis and to identify new platelet-related biomarkers of sepsis.
Interventions
OTHERBlood samples
Blood samples will be collected at inclusion.
Sponsors
National Research Agency, France
Centre Hospitalier Universitaire de Saint Etienne
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
Common inclusion criteria for ICU patients : Signed informed consent, Patient affiliated or entitled to a social security, aged over 18 years, * Criteria for blood donor voluntary : to weigh more than 50 kg * Criteria for severe sepsis group : Sepsis with failure of at least one organ, severe sepsis for less than 72 hours, sepsis with bacteria S. aureus, S. pneumoniae or E. coli. * Criteria for uninfectious inflammatory syndrome group : patients operate since less than 24 hours of hip or knee surgery, Absence of systemic infection
Exclusion criteria
* failure to participate at the study * Patients with a aspirin treatment has continued throughout severe sepsis * Patients with an appropriate antibiotic therapy for more than 72 hours * Patients with a platelet transfusion in the week before inclusion or 72 hours after a surgical gesture or the occurrence of sepsis * Patients with the platelet account is less than 30 000 per cubic millimeter the day of the sampling * Patients with a severe sepsis who had a surgical gesture previous week the inclusion * All clinical sequelae or biological at the selection * pregnant woman * Patients with a treatment by platelet aggregation has continued throughout severe sepsis
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Comparison of proportion of CD40L between the 3 groups | 1 year | Comparison of proportion of CD40L in Platelet-rich plasma (PRP) between the 3 groups by proteomics technique Luminex® stimulated by Thrombin Receptor Activator Peptide (TRAP)-6). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Comparison of proportion of CD40L between patients with severe sepsis and blood donor voluntary | 1 year | Comparison of proportion of CD40L in Platelet poor plasma (PPP) between patients with severe sepsis and blood donor voluntary by proteomics technique Luminex® stimulated by Thrombin Receptor Activator Peptide (TRAP)-6). |
| Comparison of proportion of CD40Lbetween patients with severe sepsis and patients with inflammatory syndrome without sepsis | 1 year | Comparison of proportion of CD40L in Platelet-rich plasma (PRP) between patients with severe sepsis and patients with inflammatory syndrome without sepsis by proteomics technique Luminex® stimulated by Thrombin Receptor Activator Peptide (TRAP)-6). |
| Proportion of CD40L | 1 year | Proportion of CD40L of patients with severe sepsis according to the different infection (S. aureus , S. pneumoniae, E. coli) |
Countries
France
Outcome results
None listed