Alcohol-Induced Disorders, Liver Fibroses
Conditions
Brief summary
To investigate the effects of acute alcohol challenge on the gut and liver axis.
Detailed description
To study the role of acute alcohol challenge on the intestine permeability and inflow of bacterial products to the liver in participants with alcoholic liver disease, non-alcoholic steatohepatitis and healthy controls.
Interventions
OTHEREthanol
1 milliliter per kilogram body weight adjusted for elevated body mass index
Sponsors
Odense University Hospital
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Alcoholic liver fibrosis, non-alcoholic steatohepatitis or liver-healthy individuals And all of following: * Bodymass \> 50 kg * Able to be fasting and abstinent on the day of intervention * international ratio \<1.5 and platelets \>100 x 10\^9, 7 days before the intervention day * Informed consent
Exclusion criteria
* Antibiotics during the last 4 weeks * Total alcohol abstinence * Desire of total alcohol abstinence * Other causes of liver disease suspected (viral, autoimmune, haemochromatosis, Wilsons diseases, alfa 1 antitrypsin deficiency or drug induced) * Cirrhosis * Insulin dependent diabetes mellitus * Incapable to follow the protocol instructions * Pregnancy * Primary or secondary liver cancer * Severe comorbidity * Allergic to the contrast media iohexol
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| changes in concentrations of bacterial products in the liver vein | Within 24 hours |
Secondary
| Measure | Time frame |
|---|---|
| changes in concentrations of bacterial products in the peripheral vein system | Within 24 hours |
| changes in the gut microbiome | Within 24 hours |
| changes in the liver metabolome, transcriptome and proteome | Within 24 hours |
| changes in serum-ethanol, plasma-glucose and liver biomarkers in the liver vein compared to the peripheral vein system | Within 24 hours |
Countries
Denmark
Outcome results
None listed