Obesity, Pediatric
Conditions
Keywords
Metabolic syndrome, Metabolomics, NMR spectroscopy
Brief summary
This study provides a sound basis towards a better understanding of the biochemical mechanisms behind childhood obesity and its metabolic phenotypes, which will be of great importance towards the development of more personalized prevention and treatment in future.
Detailed description
The aim of this study was to identify the plasma metabolic profile associated with childhood obesity and its phenotypes.. This cross-sectional study was set-up to examine the metabolic profile in fasting plasma samples from 65 overweight and obese (OB) children and 37 normal-weight (NW) controls aged 8 to 18 years. The obtained plasma 1H-NMR spectra were rationally divided into 110 integration regions, representing the metabolic phenotype. These integration regions reflect the relative metabolite concentrations and were used as statistical variables to construct a classification model in discriminating between OB and NW children and adolescents. In addition, two metabolic phenotypes were defined within the OB group, i.e. the metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO) phenotype which are both classified as obese but with respectively, none (MHO) or at least two components (MUO) of the metabolic syndrome.
Interventions
OTHERBlood sampling
Determine the metabolic phenotype of blood plasma by proton-NMR spectroscopy
Sponsors
Jessa Hospital
Hasselt University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
8 Years to 18 Years
Healthy volunteers
Yes
Inclusion criteria
* aged 8 to 18 * normal-weight, overweight or obese according to the International Obesity Task Force (IOTF) Body Mass Index (BMI) criteria * fasted for at least 8 hours
Exclusion criteria
* Subjects taking any medication or having serious chronic or acute illness within two weeks preceding the clinical examination,
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| metabolic phenotype of childhood obesity | day 1 | Significant metabolic changes in blood plasma of obese children and adolescents and normal-weight control subjects |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| metabolic phenotype of metabolically healthy and unhealthy obese children and adolescents | day 1 | Significant metabolic changes in blood plasma of metabolically healthy obese and metabolically unhealthy obese children and adolescents |
Outcome results
None listed