Endometriosis Ovaries, Infertility, Female
Conditions
Keywords
Endometriosis, Infertility, Telomerase activity
Brief summary
The goal of the study was the detection of telomerase activity in eutopic, ectopic endometrial tissue and in peripheral blood and finding the correlation between the telomerase activity and clinic findings. With these results, confirmation of the hypothesis regarding the endometriosis pathogenesis and endometriosis-related infertility was aimed.
Detailed description
Although distorted pelvic anatomy, impaired peritoneal, humoral and cellular functions, decreased implantation rate, oocyte and embryo quality, obstructed utero tubal connection are most researched aspects of endometriosis-related infertility, etiopathogenesis of endometriosis-related infertility still remains controversial. Endometrial cell is a unique eukaryotic somatic cell with cyclic telomerase activity. Hormone-dependent cyclic variation of endometrial telomerase activity was stated in previous studies. On the other hand telomerase, activity was shown in endometriotic lesions, whereas this activity lacks in most of the somatic cells. In this study, assessment of possible relation to increased telomerase activity in infertile patients with endometriosis was aimed. Before published studies were assessed telomerase activity in benign and malign gynecological conditions and in endometriosis tissue, as well. But evaluation of ectopic and eutopic endometrial tissue and serum telomerase activity in infertile and fertile endometriosis and healthy control subjects was not performed. Correlation of telomerase activity and endometriosis-related infertility was aimed in this study.
Interventions
PROCEDURECystectomy
Segment of cystic wall from the patients for planned cystectomy was taken after operation and was preserved at -80º Celsius for further analysis
PROCEDUREEndometrial sampling
Endometrial sampling was performed at the same operation with the planned operation for the main indication and was preserved at -80º Celsius for further analysis
PROCEDUREPeripheric blood sampling
4 cc peripheric venous blood was sampled into the EDTA(Ethylenediaminetetraacetic acid)-containing tubes during catheterization for anesthesia was preserved at 4º Celsius for further analysis
PROCEDUREOvarian tissue sampling
2cm linear tissue sample from ovarian cortex was performed at the same operation with the planned operation for the main indication and was preserved at -80º Celsius for further analysis
Sponsors
Istanbul University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Healthy volunteers
Yes
Inclusion criteria
Clinical diagnosis of Infertility Clinical and histological diagnosis of endometriosis
Exclusion criteria
Malign disorders Hormone replacement treatment Oral Contraceptive using
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Telomerase activity | through study completion, an average of 1 year | Assessment of telomerase activity with Telomeric Repeat Amplification Protocol Polymerase Chain Reaction method from tissue and blood |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Age in years | During admission to hospital for surgery | From patient anamnesis |
| Marital status (single, married, divorced, widow) | During admission to hospital for surgery | From patient anamnesis |
| Smoking status ( yes/no) | During admission to hospital for surgery | From patient anamnesis |
| BMI in kg/m2 | During admission to hospital for surgery | From patient anamnesis |
| Cystic size in cm | During operation | Macroscopically assessment during surgery |
Countries
Turkey (Türkiye)
Outcome results
None listed