Healthy Volunteers
Conditions
Brief summary
The purpose of this study is to test whether intragastrically infused fatty acid will interact with positive emotion induction and if this effect is mediated by gut hormones.
Detailed description
This study aims at the possible effect of subliminal intragastric administration of fatty acids on responses to positive emotion induction, based on our earlier findings that subjective administration of fatty acid decreases neural responses to negative mood induction (Van Oudenhove et al, J Clin Invest 2011). After screening for exclusion criteria and the participants sign the informed consent form, an intravenous catheter is placed in the forearm, and a naso-gastric tube inserted through the nose. Then the test subject is connected to the equipment in order to record the activity of the autonomic nervous system, via three electrocardiogram electrodes. By using the nasogastric tube we serve a fatty acid solution (Dodecanoic acid, fatty acid condition) or a physiological saline solution ( control condition) intragastrically. The intragastric administration of low doses of this food ingredient has already been used in several previous studies by our research group and has proven perfectly safe. A positive or neutral emotional state will be induced by the combination of looking at emotional facial expressions and listening to classical music pieces that were previously validated for this purpose; This combination of incentives has already been used in previous studies of our research group. Responses will be measured by means of validated self-report scales and measurements of the vagal tone. We also take blood samples on a regular basis by the intravenous catheter for determination of gastrointestinal hormones, given our hypothesis that this may explain the effect on emotions. After removal of the nasogastric tube, the influence of subliminal administration of fatty acids and emotional state on food intake measured by means of a validated and previously drink test used in which participants ad libitum to drink a chocolate milkshake while the emotion-induction by means of classical music pieces continue. Finally, the intravenous catheter is removed.
Interventions
DIETARY_SUPPLEMENTDodecanoic acid
The middle chain saturated fatty acid that can be widely found in daily food.
BEHAVIORALPositive emotion
Positive emotion induced by facial expressions and classic music.
DIETARY_SUPPLEMENTsaline
saline
BEHAVIORALneutral emotion
Neutral emotion induced by facial expressions and classic music.
Sponsors
Universitaire Ziekenhuizen KU Leuven
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy women * age \> 18 and \< 60. * Body Mass Index (BMI) of 19-25 kg/m2 (no weight change of more than 5% of body weight in the past three months).
Exclusion criteria
* chronic medical illness affecting the gastrointestinal, endocrine, cardiovascular or nervous system (risk of) pregnancy history of upper gastrointestinal surgery history of behavioral, therapeutic or surgical treatment aiming at or leading to weight loss/gain psychiatric disorders, including (history of) depression or eating disorders alcohol abuse or dependence regular use of medication, except for oral contraception use of any drugs, including cannabis, during the past 6 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| time evolution of VAS from the beginning until the end of a study visit | -10min 0min 10min 20min 30min 40min | visual analogue scale for appetite related sensations |
| time evolution of gut hormones from the beginning until the end of a study visit | -10min 10min 20min 30min 40min | — |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| evolution of heart rate variability | -20min 20min | — |
| hedonic food intake | 40min | amount of milkshake drunk |
Countries
Belgium
Outcome results
None listed