Dental Caries
Conditions
Keywords
Carious dentin, Photodynamic Therapy
Brief summary
The aim of this study is to assess the clinical effect of aPDT on deep caries in permanent teeth. Half of the deep caries will receive aPDT disinfect in restorative treatment procedure, while the other half will receive sodium 0.9% saline in restorative treatment procedure.
Detailed description
The aim of the proposed study is to assess the clinical effect of antimicrobial photodynamic therapy (aPDT) using LED on dentin with deep caries in permanent teeth. Permanent teeth with deep carious lesions on the occlusal surface indicated for restorative treatment will be randomly allocated to two groups: Group 1 -Control after incomplete caries removal, washed with sodium 0.9% saline; Group 2 - PDT + CH after incomplete caries removal, disinfect the residual dentin with PDT(LED + O-Toluidine Blue). Dentin samples will be collected before and after disinfect for microbiological analysis. Then the teeth will be capped with CH and restored using resin with clinical and radiographic follow up at 6, 12 and 24 months.
Interventions
PROCEDURELocal anesthesia and rubber dam isolation
Local anesthesia was performed. Tooth was isolated using a rubber dam isolation in order to collect the dentin samples.
PROCEDUREIncomplete caries removal and microbiological samples collection
Partial carious tissue was removed with conventional dentinal curette and collection of dentin with a spoon (1#) was performed.
PROCEDUREDentin washed with sodium 0.9% saline
The remaining dentin was dipped in sodium 0.9% saline for 2min.
PROCEDUREAntimicrobial Photodynamic Therapy
The remaining dentin was disinfected with aPDT for 2 min.
PROCEDUREMicrobiological samples collection and Indirect pulp treatment
A new dentin collection was performed after this treatment at another site of the cavity. Then teeth were conventionally restored using the following materials: calcium hydroxide lining material and resin (Z350,3M).
Sponsors
Fujian Medical University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* Good general health, without syndromes or chronic systemic diseases * Permanent tooth with deep carious lesion (2/3 of dentin) without pain symptomatology and compatible with reversible pulpits
Exclusion criteria
* Refused to sign the informed consent document * Allergic to photosensitizer * Unlikely to be able to comply with the study procedures
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Quantification of bacteria by colony counting | 2 days after dentin collection | Quantification of total bacteria |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Tooth Pain | 1 day and 6, 12, 24 months | Patients reported the presence or absence of tooth pain |
| Restoration Quality | 1 day and 6, 12, 24 months | The quality of restoration was evaluated through the absence of radiographic radiolucent area |
Countries
China
Contacts
Primary ContactXiuqing Wang, Bachelor
Outcome results
None listed