Polar Body Removal Before ICSI for Oocyte Activation in Previous Fertilization Failure Cases

Polar Body Removal Before ICSI as a Method of Oocyte Activation in Previous Fertilization Failure Cases

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02913781

Acronym

PBRBICSI

Enrollment

Registered

2016-09-26

Start date

2016-02-29

Completion date

2018-01-31

Last updated

2018-09-11

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Fertilization

Keywords

Polar body removal, ICSI, Fertilization failure

Brief summary

Cases which undergone ICSI before with failure of fertilization will do another ICSI cycle with removal of the polar body just before the ICSI procedure and sperm injection at the site of polar body removal. This study group will be evaluated with another group with the classic ICSI procedure

Detailed description

Patients with previous fertilization failure will be divided into 2 groups: Group 1 (control): Will undergo classic ICSI procedure in half the MII oocyte of the same case. Group 2 (study group): The other half of the MII oocyte of the same case will undergo: 1. Polar body removal. 2. ICSI procedure with injection of the spermatozoon in the same zona opening (done by laser for polar body removal). Results: Will be collected with evaluation of number of 2PN zygotes, Day 1, Day 2, Day 3, Day 5 embryos in all the cases.

Interventions

PROCEDUREpolar body removal using zona drilling by laser then suction by injecting pipette

zona drilling with laser followed by polar body removal then ICSI procedure

PROCEDUREClassic ICSI

Classic ICSI case was done be injecting sperm inside Oocyte while polar body is already existed at 6 or 12 O'clock position.

Study design

Allocation

Intervention model

SINGLE_GROUP

Primary purpose

TREATMENT

Masking

NONE

Eligibility

Sex/Gender

FEMALE

Age

20 Years to 40 Years

Healthy volunteers

Yes

Inclusion criteria

* previous fertilization failure * previous fertilization arrest

Exclusion criteria

* NO MII oocyte * Refusal of the procedure by the couple

Design outcomes

Primary

Measure	Time frame	Description
fertilization rate of the zygotes	16-20 hours post ICSI	fertilization check is done under inverted microscopy by 2PN check

Secondary

Measure	Time frame	Description
division rate of the embryos by the number of the blastomeres.	Day 2 & 3 & 5	Detection of the number of the blastomeres and quality of the embryos under inverted microscopy. At day 5, detection of the rate of the blastocyst formation and quality of the embryos.

Countries

Egypt

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026