Salivary Bacterial Associations to Short Term Discontinuation of Self-performed Oral Hygiene

Does Short Term Discontinuation of Self-performed Oral Hygiene Procedures Influence the Composition of the Salivary Microbiota?

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02913235

Enrollment

Registered

2016-09-23

Start date

2016-09-30

Completion date

2017-01-02

Last updated

2017-02-03

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Mouth Diseases

Keywords

biofilm, saliva, bacteria, oral hygiene

Brief summary

The purpose of the present investigation is to record and compare bacterial compositions in supragingival plaque samples and saliva samples in subjects discontinuing regular oral hygiene for 10 days. The hypothesis is that the composition of the salivary microbiota might reflect local bacterial alterations in relation to discontinuation of oral hygiene.

Detailed description

35 orally healthy individuals will be enrolled in this study, in which they will discontinue regular oral hygiene procedures for a period of 10 days. Clinical registrations and collection of microbiological samples (supragingival and saliva) will be performed at baseline and 4, 7 and 10 days after discontinuation of regular oral hygiene, and will be repeated 14 days after regular oral hygiene has been resumed.

Interventions

PROCEDUREOral hygiene discontinuation group

Discontinuation of regular oral hygiene for 10 days

Study design

Allocation

Intervention model

SINGLE_GROUP

Primary purpose

DIAGNOSTIC

Masking

NONE

Eligibility

Sex/Gender

ALL

Age

18 Years to 40 Years

Healthy volunteers

Yes

Inclusion criteria

* Oral and systemically healthy individuals

Exclusion criteria

* Periodontitis * Dental caries * Systemic diseases or medications with known associations to oral health * Antibiotics within the latest 6 month

Design outcomes

Primary

Measure	Time frame	Description
Comparison of changes in α-diversity in saliva samples before and after discontinuation of regular oral care	10 days of discontinuation of regular oral care and 14 days after resuming regular oral care	Comparison of α-diversity in saliva samples before and after discontinuation of regular oral hygiene

Secondary

Measure	Time frame	Description
Comparison of species specific changes in saliva samples before and after discontinuation of regular oral care	10 days of discontinuation of regular oral care and 14 days after resuming regular oral care	Comparison of relative abundance of bacterial species in saliva samples at baseline to samples collected 10 days after discontinuation of regular oral hygiene and to samples collected 14 days after oral hygiene procedures has been resumed

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 26, 2026