Rheumatoid Arthritis
Conditions
Keywords
rheumatoid arthritis, Porphyromonas gingivalis
Brief summary
Rheumatoid arthritis (RA) is the most frequent joint inflammatory disease inducing joint destruction strongly correlated with anti-citrullinated protein antibodies citrullinated anti-peptide (ACPA). Precise RA etiology remained unclear with a described association between RA and periodontal disease. The missing link could be a gram-negative anaerobic bacterium called Porphyromonas gingivalis (P. gingivalis), which is heavily involved in periodontal disease and able to induce gingival citrullinated protein via an enzyme called peptidyl arginine deiminase (PAD). DNA of P. gingivalis was detected in synovial tissue and in peripheral blood mononuclear cell. To improve knowledge of P. gingivalis involvement in RA, the investigators will compare its effect on various cell type with comparison with another oral bacteria Provatella intermedia.
Interventions
PROCEDUREsynovial tissue
synoviocytes from synovial tissue
PROCEDUREblood sample
Sponsors
Centre Hospitalier Universitaire de Saint Etienne
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Age \>18 ans * RA or osteoarthritis with joint surgery planned * Informed consent approved and signed
Exclusion criteria
* Pregnancy
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Comparison of genetic signature after induction by P. gingivalis and P. intermedia on synoviocytes | day 1 |
Secondary
| Measure | Time frame |
|---|---|
| Comparison of specific transcriptome profile induced by P. gingivalis compared to P. intermedia on peripheral blood mononuclear cells (PBMC) and gingival fibroblasts | day 1 |
| Comparison of transcriptome profile between P. gingivalis and P. intermedia on RA synovial cells. | day 1 |
| Comparison of the transcriptomic profile between RA and osteoarthritis synoviocytes | day 1 |
Countries
France
Outcome results
None listed