Side of Embryo Biopsy Interfering Implantation Potential

Effect of Embryo Biopsy Side on Post Biopsy Blastocyst Structurs and Implantation

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT02902614

Enrollment

100

Registered

2016-09-16

Start date

2015-01-31

Completion date

2018-12-02

Last updated

2019-01-15

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Preimplantation Diagnosis

Keywords

embryo biopsy, preimplantation genetic testing, animal pole, vegetal pole, day 3 embryo biopsy, embryo implantation, Blastomeres

Brief summary

Selection of side of embryo biopsy that will not interfering with implantation power of developed blastocyst embryo during biopsy procedure arranged for PGT.

Detailed description

During process of embryo biopsy, it was indicated to avoid contacting cells closer to inner cell mass as it can decrease the quality of embryo and its structural composition. However, recommendation for collection from the trophectoderm (TE) side is indicated. Absence of differentiation during cleavage-stage or morula stage at day 3 and day 4 respectively, will present the needful to focus a specific location for embryo biopsy during pre-blastocyst stages for embryo going to do genetic testing before implantation or embryo transfer. In articulation around the second polar body or the animal pole of embryo, collection of the cell from the vegetal pole in crospondence to avoide collection form the animal pole is to be compare the results of implantation for biopsy done using the animal pole side or the random non organized biosy procedure.

Interventions

PROCEDUREVegetal Pole blastomer embryo

for day 3 embryo biopsy / cleavage stage

PROCEDUREAnimal Pole blastomer embryo

for day 3 embryo biopsy / cleavage stage

Study design

Allocation

NON_RANDOMIZED

Intervention model

CROSSOVER

Primary purpose

SUPPORTIVE_CARE

Masking

NONE

Eligibility

Sex/Gender

ALL

Healthy volunteers

Inclusion criteria

* grade 1 or 2 embryos at eight cell stage or 8-1/ 8-2 embryo quality.

Exclusion criteria

* grade 3 embryo at day 3, or 8-3 embryos.

Design outcomes

Primary

Measure	Time frame	Description
blastocyst location of ICM at day 5	2 months	describing the location of the inner cell mass (ICM) in location to the zona peluceda (ZP) drilling opening.

Secondary

Measure	Time frame	Description
Implantation power	2 months	calculating the pregnancy rate for cases after biopsy.

Countries

Bahrain, Egypt

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026