Acute Gastroenteritis, Gastroenteritis
Conditions
Keywords
Vaccine, Polymorphism
Brief summary
The GASTROVIM research explores the links between individual genetic susceptibility, genetic variability of rotavirus strains and effectiveness of immunization with the rotavirus vaccination: a clinical investigation to assess glycan attachment specificity, toward rotavirus vaccine improvement.
Detailed description
The work will be carried out in France and in a tropical area of population with diverse geographical origins: the Guyana populated with Native Americans, people of European descent, people of Asian (Hmong) and a large source population African. The first aim of the project will be to characterize the specificity of the VP8 \* HBGA of RotaTeq and Rotarix vaccines in order to ensure their binding characteristics glycans are similar to those of recent P8 clinical strains circulating in France and were maintained in the culture passages. The second objective will be to validate the impact of recently described polymorphisms HBGAs on susceptibility to infection by RVA through GASTROVIMc prospective clinical research. The third objective will be to determine the relative role of recognition and HBGAs ganglioside in the initial attachment and viral entry. The expression of HBGAs and ganglioside by permissive cell lines in culture human stem RVA be manipulated to define the role of glycans in attachment and infection.
Interventions
BIOLOGICALrotavirus rapid screen test
According to routine care
GENETICpolymorphism exploration
from an oral swab collected after parents consent
Sponsors
Nantes University Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
No minimum to 16 Years
Healthy volunteers
No
Inclusion criteria
* 0 to 16. * giving consent to participate if applicable (\>6yo) * Parents informed and written consent obtained * admitted in paediatric emergency for wether an acute gastroenteritis (case group) or anything else than an infection or gastroenteritis (control group)
Exclusion criteria
* case group: rotavirus rapid screen test negative; viral gastroenteritis other than rotavirus; bacterial gastroenteritis * control group: Family and / or patient unable to full informed consent to research; Infectious Pathology context in general.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Absence / Presence of non-secretory polymorphisms FUT2- and Lewis Negative FUT3- | 6 months after inclusion |
Secondary
| Measure | Time frame |
|---|---|
| Population frequency of new protective genotypes to rotavirus infection | Six months after inclusion |
| Absence / presence of genetic polymorphisms --other (Including FUT2- and FUT3-) | Six months after inclusion |
| Absence / presence of different viral strains Rotavirus (RVA) involved in the acute gastroenteritis episodes in French Guiana | Six months after inclusion |
| Absence / presence of various HBGA polymorphisms involved in acute gastroenteritis episodes in French Guiana | Six months after inclusion |
| population frequency of different etiologies of acute gastroenteritis viral, bacterial and / or parasitic (Cayenne) | Six months after inclusion |
Countries
France
Outcome results
None listed