Diaphragm Dysfunction, Mechanical Ventilation
Conditions
Brief summary
This non-interventional study focuses on mechanical ventilation used in intensive care unit to supplement ventilatory function in patients. Mechanical ventilation can paradoxically be at the origin of complications that can be life-threatening in patients. This muscular pathology is called ventilation-induced diaphragmatic dysfunction (DDIV). Diaphragmatic muscle collected during a digestive surgery for a benign or malignant tumor of the liver requiring surgical excision in contact with the diaphragm from the care will be conserved. The diaphragm biopsy from the care will be retained for biobanking to obtain myoblast in culture which will differentiate in Diaphragm fiber. Then these fibers will be submitted under mechanical stress condition similar to those imposed in vivo by mechanical ventilation to validate in human a model in vitro of diaphragm dysfunction induced by mechanical ventilation. Then the second part of the study will be to evaluate with this model, the efficiency of an antioxidant therapy.
Interventions
None listed
Sponsors
University Hospital, Montpellier
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
30 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
* age between 30 and 65 years old * patient treated by the digestive surgery department of Montpellier University Hospital for a benign or malignant tumor of the liver requiring surgical excision in contact with the diaphragm * Non-smoker patient for more than 6 months * patient clinically stable at the time of the study, ie not requiring any treatment
Exclusion criteria
* Patients undergoing treatment with antibiotics or corticosteroids, or recently within the last 4 months * Patients with a body mass index \> 30 * Patients with any criteria that may in themselves impair respiratory muscle function such as chronic obstructive pulmonary disease, heart failure, systemic infection, neuromuscular pathology, psychiatric pathology or metabolic disorder. Patients with coagulopathy or thrombocytopenia.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Myoblast differentiation capacity | 24 month | Description: evaluation of ROS (reactive oxygen species) production by confocal microscopie of diaphragm fiber submitted in vitro by mechanical stress. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Myoblast proliferation capacity | 24 month | Description: Evaluation of diaphragm myoblast differenciation capacity in vitro under different concentration of antioxydant. |
Countries
France
Outcome results
None listed