Beta-Lactams Allergy, Hypersensitivity
Conditions
Brief summary
Beta-lactam antibiotics include penicillin and cephalosporins and are among the most prescribed antibiotics. This category of drugs is the most involved in immediate allergic manifestations with 2% reactions in treated subjects and a fatal outcome in 1/50000 treatments. Reactions are IgE-mediated and have a considerable but unknown genetic origin, revealed by studies in groups of different ethnical origins in the same geographical region. There are also some families with a high frequency of allergic reactions without identified Mendelian inheritance. The purpose of this study is to identify predictive risk factors associated to immediate allergic reactions against beta-lactam antibiotics with a pangenomic approach. A secondary purpose is to identify rare predictive factors with homozygosity mapping and exome sequencing in various families with high risk of allergy to beta-lactam antibiotics.
Interventions
Sponsors
Central Hospital, Nancy, France
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
* Informed consent * Allergy group: Immediate allergic reaction (less than 2 hours after drug administration) to an antibiotic of penicillin or cephalosporin groups * Allergy group: Positive skin test (intradermal test) with a non-irritant dilution of antibiotic of penicillin or cephalosporin groups
Exclusion criteria
* Refusal of participation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Analysis of genetic polymorphisms predictive of risk of beta-lactam immediate allergy | day 0 | Common (\>10%) or rare (1-10%) genetic polymorphisms in patients with beta-lactam immediate allergy |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Analysis of allelic homozygosity linked to allergen response, in more than 2 allergic individuals of the same family | day 0 | Allelic homozygosity in one or more regions of genome with one or more genes involved in allergen response, in more than 2 individuals affected by beta-lactam allergy in the same family |
| Severity of allergic reaction | day 0 | evaluated during clinical examination |
| Reactivity to skin test | day 0 | — |
| Serum concentration of specific IgE | day 0 | — |
Outcome results
None listed