Head and Neck Cancer
Conditions
Keywords
circulating tumor cell, CellSearch™ method, RT-PCR
Brief summary
The dissemination of individual tumor cells is a common phenomenon in solid cancers. Detection of tumor cells in peripheral blood circulating tumor cells (CTC) in nonmetastatic situation is of high prognostic significance. The objective of our study was to detect circulating tumor cells in two different method in patient with head and neck squamous cell carcinoma .
Interventions
OTHERCirculating Tumor Cells
Patients with stage III and IV head and neck squamous cell carcinoma undergoing surgery were enrolled in this study 5 and 7.5 ml of peripheral venous blood will be taken before the surgery, just at the end of surgery and one week after surgery Analysis was performed by RT-PCR and the CellSearch™ method using immunomagnetic and fluorescence approaches. Patients will be followed every 3 months for two years.
Sponsors
Institut de Cancérologie de Lorraine
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Age ≥ 18 years * Patient with head and neck squamous cell cancer stage III or IV histologically proven * Patient naive to any prior therapy * Patient with operate cancer and resectable tumor * Hemoglobin ≥ 10g / dL * Performance status ≤ 2 * Ability to provide written informed consent * Patient's legal capacity to consent to study participation
Exclusion criteria
* Other previous histology tumors * Medical conditions or acute or chronic severe psychiatric disorders * Deprived of liberty or under supervision
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Circulating tumor cells in peripheral blood during head and neck surgery | 7 days | The number of circulating tumor cells detected per milliliter of peripheral blood will be evaluated on the day before, the day of surgery and 7 days after the surgery. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Comparison between RT-PCR and CellSearch™ method | 1 day | The comparison between RT-PCR and CellSearch™ method will be the positivity sample rate |
| Prognostic marker of relapse | 2 years | Local recurrence, regional or metastatic head and neck cancer in the two years |
Countries
France
Contacts
PRINCIPAL_INVESTIGATORMASTRONICOLA Romina, MD
Institut de Cancérologie de Lorraine
Outcome results
None listed