Wound
Conditions
Brief summary
The purpose is to determine intrinsic properties of various immunohistochemical markers (FVIIIra, CD15, CD30, tryptase, TNFα, IL-1β, TGFα et TGFβ1) for diagnosis of vital wound, alone and in association (evaluation of sensibility with surgery wounds and evaluation of specificity with post-mortem wounds). Secondary purposes are to measure the minimum time to obtain a positive labeling in vital wounds, and to evaluate inter-observer reproducibility of vitality diagnosis with different markers. Expression of microRNA miR 9, miR 21 et miR 198 will be also studied.
Interventions
OTHERAbdominoplasty with exeresis of cutaneous tissue
OTHERCollection of cutaneous tissue
Sponsors
Central Hospital, Nancy, France
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
Alive persons: \- Abdominoplasty Deceased persons: \- Medico-scientific autopsy for diagnosis
Exclusion criteria
Alive persons: * Fragmented sample with non-visible banks * Cutaneous pathology Deceased persons: * Persons under protection * Medico-legal obstacle * Cutaneous pathology
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Sensibility of markers for vitality diagnosis | baseline |
| Specificity of markers for vitality diagnosis | baseline |
Secondary
| Measure | Time frame |
|---|---|
| Expression level of miR 9 by qRT-PCR | baseline |
| Minimum time to obtain a labeling (between incision and devascularization) | baseline |
| Expression level of miR 198 by qRT-PCR | baseline |
| Expression level of miR 21 by qRT-PCR | baseline |
| Coefficient of inter-observer correlation of vitality diagnosis (for reproducibility analysis) | baseline |
Countries
France
Contacts
Primary ContactLaurent MARTRILLE
Outcome results
None listed