Purpura, Thrombocytopenic, Idiopathic, Regulatory B Cells
Conditions
Brief summary
The chronic immune thrombopenia is an autoimmune disease caused by B cells. These cells produce anti platelets and megakaryocytes antibodies. Some B cells, named regulatory B cells, are known to control other cells. Their action in chronic immune thrombopenia is actually unknown.
Detailed description
The chronic immune thrombopenia is an autoimmune disease caused by B cells. These cells produce anti platelets and megakaryocytes antibodies. Some B cells, named regulatory B cells, are known to control other cells. Their action in chronic immune thrombopenia is actually unknown. In our cohort of chronic immune thrombopenia and controls, we realized a flow cytometry analyze of B and T cells and a co-culture to determine regulatory B cells functionality.
Interventions
BIOLOGICALBiological tests
From the blood: B and T cells extraction then co-culture B and T cells and characterization of lymphocytes by flow cytometry
Sponsors
University Hospital, Brest
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* Purpura, Thrombocytopenic, Idiopathic, chronic phase (\>12 months) * no treatment or thrombopoietin receptor agonists
Exclusion criteria
* \< 18 years * Purpura, Thrombocytopenic, Idiopathic, acute phase * pregnancy * others causes of thrombopenia
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Regulatory B cells functionality | 1 week | inhibition of T cells proliferation with co-culture B and T cells |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Cytometry immunophenotyping of B and T cells | 1 week | characterization of B and T cells by flow cytometry |
Countries
France
Outcome results
None listed