Chromosome Abnormalities, Genetic Diseases, Inborn, Mutation
Conditions
Keywords
PGS, PGD, free embryo DNA, NGS, PGT, PGT-a, PGT-m, PGT-HLA
Brief summary
In the way for developing and optimizing protocol to be used as non- invasive methodology used as routine testing for PGS. This protocol is to be adapted to replace the using of life embryo cells for genetic testing and aneuploidy study as well as for any type of genetic testing including single gene disorder or HLA typing or study.
Detailed description
Compare 100 embryos studied using NGS for chromosomal aneuploidies, and compare the results came from free media DNA with this results.
Interventions
PROCEDURENGS reading for Media Free DNA
NGS reading for Media Free DNA instead of blstomer biopsy
Sponsors
Ibn Sina Hospital
Sadat City University
Al Baraka Fertility Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
20 Years to 45 Years
Healthy volunteers
No
Inclusion criteria
* all abnormal/aneuploidy embryos rejected for embryo transfer.
Exclusion criteria
* all embryos had unsuccessful diagnosis with blastomere biopsy.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| DNA isolation | 2 years | reading amplified DNA using NGS |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| NGS evaluation | 2 years | Free DNA isolation and amplification using WGA |
| Comparison with cell reading using NGS | 2 years | compare the obtained result with the results provided collected for the same embryo using its blastomer for PGS |
Outcome results
None listed