Obesity
Conditions
Brief summary
The target of rapamycin complex 2 (TORC2) is an evolutionarily conserved serine/threonine protein kinase that controls growth and metabolism. In mammals (including humans), mammalian TOR complex 2(mTORC2) contains mammalian TOR (mTOR), RICTOR, mSIN1 protein, and mLST8 gene. In an animal model, the adipose-specific rictor knockout (AdRiKO) mouse, systemic insulin resistance, hepatic steatosis, and cardiovascular dysfunction develop upon high fat diet (HFD)-induced obesity or aging. To find a molecular link between adipose mTORC2 and systemic insulin resistance, investigators have already performed transcriptomics and proteomics analysis on visceral white adipose tissue in a mouse model. The aim of the study is to confirm a molecular link between adipose mTORC2 and systemic insulin resistance in humans.
Interventions
Sponsors
University Hospital, Basel, Switzerland
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Obese volunteers (BMI \> 35kg/m2) aged 18 to a maximum of 60 years, males and females; insulin-resistant, scheduled for elective bariatric surgery. * Normal weight patients (BMI \< 27kg/m2) aged 18- max. 60 years, males and females; insulin-sensitive, scheduled for elective surgery.
Exclusion criteria
* Known diabetes mellitus * Chronic inflammatory disease (inflammatory bowel disease, rheumatoid disease, cancer) * Acute inflammatory disease * Known renal disease: kidney failure * Pregnancy: * history of gastrointestinal surgery with major changes to the gastrointestinal tract (removal of stomach, any GI-bypass) * Substance abuse, alcohol abuse * Inability to follow procedures due to psychological disorders, dementia * insufficient knowledge of project language (German)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| mTORC2 activity in adipose tissue measured by immunoblot | single time point at surgery | measurement of mTORC2 activity in adipose tissue once at surgery |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Mcp1 messenger ribonucleic acid (mRNA) level in adipose tissue measured by quantitative polymerase chain reaction (PCR) | single time point at surgery | measurement of Mcp1 messenger ribonucleic acid (mRNA) level in adipose tissue once at surgery |
Other
| Measure | Time frame | Description |
|---|---|---|
| Insulin resistance measured with fasting plasma insulin and fasting glucose | single time point at surgery | measurement of fasting plasma insulin and fasting Glucose once at surgery |
Outcome results
None listed