Overweight
Conditions
Keywords
Glucagon-Like Peptide 1, Carbohydrates, Gastric bypass, Bariatric Surgery, Overweight
Brief summary
To investigate the secretion of gut hormones, in particular glucagon-like peptide 1 (GLP-1), after ingestion of glucose and fructose as separate monosaccharides or combined in the disaccharides sucrose and isomaltulose and the effect of acarbose in gastric bypass operated subjects and BMI-matched controls.
Detailed description
Disaccharides such as sucrose or isomaltulose (also called palatinose) require digestion into monosaccharides (both are digested to glucose and fructose) by enzymes located in the brush border of the small intestine to be absorbed. The study will investigate the influence of carbohydrate digestion on gut hormone secretion.
Interventions
OTHERGlucose + Fructose
Ingestion of the separate monosaccharides glucose (25 g) and fructose (25 g) dissolved in 200 mL of water. The load provides 0.1385 mol glucose and 0.1385 mol fructose.
OTHERSucrose
Ingestion of the disaccharide sucrose (47.5 g) dissolved in 200 mL of water. The load provides 0.1385 mol glucose and 0.1385 mol fructose.
OTHERIsomaltulose
Ingestion of the slowly digestable disaccharide isomaltulose (47.5 g) dissolved in 200 mL of water. The load provides 0.1385 mol glucose and 0.1385 mol fructose.
OTHERSucrose + Acarbose
Ingestion of the disaccharide sucrose (47.5 g) and the alpha glucosidase inhibitor acarbose dissolved in 200 mL of water. The load provides 0.1385 mol glucose and 0.1385 mol fructose.
Sponsors
University of Copenhagen
Hvidovre University Hospital
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
Gastric bypass operated patients: Inclusion Criteria: * uncomplicated gastric bypass surgery performed minimum 12 months prior to study
Exclusion criteria
* Type 1 or 2 diabetes mellitus prior to or after gastric bypass surgery * Pregnancy or breastfeeding * Haemoglobin levels below 6,5 mM Healthy control subjects:
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Within group difference in GLP-1 secretion (evaluated by iAUC). | 0-240 min following carbohydrate ingestion | Comparison of the GLP-1 responses (evaluated by iAUC) induced by the different carbohydrate loads. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Between group comparison of differences in GLP-1 secretion (evaluated by iAUC). | 0-240 min following carbohydrate ingestion | Differences in GLP-1 secretion (evaluated by iAUC) induced by the different carbohydrate loads in gastric bypass operated patients compared to differences in BMI-matched control subjects. |
| Responses (evaluated by iAUC) of glucose within and between groups. | 0-240 min following carbohydrate ingestion | — |
| Responses (evaluated by iAUC) of insulin within and between groups. | 0-240 min following carbohydrate ingestion | — |
| Responses (evaluated by iAUC) of C-Peptide within and between groups. | 0-240 min following carbohydrate ingestion | — |
| Responses (evaluated by iAUC) of peptide YY (PYY) within and between groups. | 0-240 min following carbohydrate ingestion | — |
| Between group comparison of GLP-1 secretion (evaluated by iAUC). | 0-240 min following carbohydrate ingestion | GLP-1 secretion (evaluated by iAUC) in gastric bypass operated patients compared to BMI-matched control subjects for each of the carbohydrate loads. |
| Responses (evaluated by iAUC) of glucagon within and between groups. | 0-240 min following carbohydrate ingestion | — |
| Responses (evaluated by iAUC) of oxyntomodulin within and between groups. | 0-240 min following carbohydrate ingestion | — |
| Responses (evaluated by iAUC) of cholecystokinin (CCK) within and between groups. | 0-240 min following carbohydrate ingestion | — |
| Responses (evaluated by iAUC) of bile acids within and between groups. | 0-240 min following carbohydrate ingestion | — |
| Responses (evaluated by iAUC) of Gastric Inhibitory Peptide (GIP) within and between groups. | 0-240 min following carbohydrate ingestion | — |
Countries
Denmark
Outcome results
None listed