Crohn Disease
Conditions
Keywords
genomics, epigenomics, recurrence
Brief summary
The purpose is to search for enterocyte-specific or non-specific molecular signature of post-operating recurrence of Crohn's disease in transcriptome (measurement of gene expression through number of mRNA copies transcribed for each gene) and in methylome. Secondary objectives are to compare expression and methylation profiles according to: * treatment response * morphological (endoscopic and/or radiological) and clinical post-operating recurrence.
Detailed description
This aim of this study is to search for new biomarkers predictive of post-operating recurrence. At present, there are no biomarkers predictive for post-operating recurrence and administration of a post-operating medical treatment to prevent recurrence is based on biological, endoscopic and imagery data. Predictive biomarkers could allow the identification of patients with high risk of post-operating recurrence and administration of a systematic prophylactic post-operating treatment and at the same time avoiding the prescription of this treatment to patients with low risk of post-operating recurrence.
Interventions
PROCEDUREIleocolic resection
Sponsors
Central Hospital, Nancy, France
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Patient with inflammatory bowel disease diagnosis based on clinical (abdominal pain, diarrhea and/or dysenteric syndrome, mostly muco-hemorrhagic, perianal injury, with sometimes general signs and /or systemic manifestations ), biological (inflammatory syndrome, deficiencies…), radiological (entero-MRI), endoscopic (presence of granulomatous lesions) and/or histological (epithelioid and giant cellular granuloma) data * Patient undergoing ileocolic resection * Patient affiliated to French social security
Exclusion criteria
* Patient refusing sampling * Patient with undetermined colitis * Patient with non-confirmed diagnosis of inflammatory bowel disease * Patient with inflammatory bowel disease not undergoing ileocolic resection
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Gene expression profile in intestinal operative specimens | up to 2 months (from resection) |
| Methylation profile in intestinal operative specimens | up to 2 months (from resection) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Digestive symptoms | 6, 12, 24 months (after resection) | such as abdominal pain, Koenig syndrome (abdominal pain relieved by flatus or feces expulsion), diarrhea |
| Radiological classification of recurrence with entero-MRI performed for post-operating follow-up of patients | 6, 12, 24 months (after resection) | Koilakou score, recurrence when score \>=1 |
| Evaluation of recurrence with endoscopy performed for post-operating follow-up of patients | 6, 12, 24 months (after resection) | Rutgeerts criteria (recurrence when score \>=i1) |
Contacts
Primary ContactAdeline GERMAIN
Outcome results
None listed