Diabetes, Obesity
Conditions
Brief summary
This pilot study is being conducted to evaluate whether consumption of diet soda sweetened with sucralose and acesulfame-potassium leads to changes in the gut microbiota. Following a one week run-in period, healthy, young adults are randomized to consume either diet soda or carbonated water (control) three times per day for one week. Stool and urine samples are collected at baseline, following the run-in, and after the one-week intervention.
Interventions
OTHERcommercially-available diet soda
Subjects are asked to consume diet soda three times daily for one week.
OTHERcarbonated water (control)
Subjects are asked to consume carbonated water three times daily for one week.
Sponsors
George Washington University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
SINGLE (Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy adults aged 18-35 years * Consume less than 1 food/beverage with non-nutritive sweeteners per month * Able and willing to consume diet soda three times daily for 1 week
Exclusion criteria
* Recent diet or significant weight change * Antibiotic use in the past 3 months * Diarrhea in the past two weeks * Use of medications known to affect metabolism or weight * Weight \< 50 kg (110 lbs.) * History of metabolic complications including high blood sugar, elevated cholesterol, or liver disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Taxa abundance before and after one-week of three times daily diet soda consumption | one week | 16S rRNA and metagenomics sequencing will be used to determine microbial composition of stool samples before and after the one week intervention. |
Outcome results
None listed