Regeneration of Bronchial Epithelium During Chronic Obstructive Pulmonary Disease

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT02873988

Acronym

EPITHREGENCOPD

Enrollment

Registered

2016-08-22

Start date

2012-03-31

Completion date

2014-05-31

Last updated

2016-08-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

COPD

Brief summary

Airway epithelium integrity is essential to maintain its role of mechanical and functional barrier. Recurrent epithelial injuries require a complex mechanism of repair to restore its integrity. In chronic obstructive pulmonary disease (COPD), an abnormal airway epithelial repair may participate in airway remodelling. The objective was to determine if airway epithelial wound repair of airway epithelium is abnormal in COPD.

Detailed description

Patients scheduled for lung resection were prospectively recruited. Demographic, clinical data and pulmonary function tests results were recorded. Emphysema was visually scored and histological remodelling features were noted. Primary bronchial epithelial cells (BEC) were extracted and cultured for wound closure assay. The investigators determined the mean speed of wound closure (MSWC) and cell proliferation index, matrix metalloprotease (MMP)-2, MMP-9 and cytokines levels in supernatants of BEC 18 hours after cell wounding. In a subset of patients, bronchiolar epithelial cells were also cultured for wound closure assay for MSWC analyze.

Interventions

OTHERbronchial epithelial cell culture

Study design

Observational model

CASE_CONTROL

Time perspective

CROSS_SECTIONAL

Eligibility

Sex/Gender

ALL

Age

40 Years to No maximum

Healthy volunteers

Inclusion criteria

6 Patients scheduled for lung resection for cancer

Exclusion criteria

* Patients with asthma, cystic fibrosis, bronchiectasis or pulmonary fibrosis were excluded

Design outcomes

Primary

Measure	Time frame
Mean speed of wound closure (MSWC) of wounded bronchial epithelial cell culture obtained from COPD and control patients	hour 18

Secondary

Measure	Time frame
Cell proliferation index in supernatants of bronchial epithelial cells 18 hours after cell wounding.	hour 18
Matrix metalloprotease (MMP)-2 level in supernatants of bronchial epithelial cells 18 hours after cell wounding.	hour 18
Matrix metalloprotease (MMP)-9 level in supernatants of bronchial epithelial cells 18 hours after cell wounding.	hour 18
Cytokines levels in supernatants of bronchial epithelial cells 18 hours after cell wounding.	hour 18

Countries

France

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026