Healthy
Conditions
Keywords
Increased Metabolic Requirement
Brief summary
Protein requirements in active individuals have been suggested to be greater than the current recommended dietary allowance (RDA). Nutritional requirements for dietary amino acids in adolescents have traditionally been determined utilizing the nitrogen balance technique, which is prone to underestimating protein requirements. As a result, there is a need to re-evaluate recommendations in order to characterize how dietary amino acid needs may be modulated by physical activity. Recent studies using the minimally invasive indicator amino acid oxidation (IAAO) technique have suggested that protein requirements in young men are at least 50% higher than the RDA based on nitrogen balance data. The purpose of this study is to measure the protein requirement in adolescent, males using the IAAO technique in the presence of a variable intensity exercise stimulus.
Interventions
DIETARY_SUPPLEMENTAmino Acid Intake
Amino acid intakes will be varied at 0.2-2.67 g/kg/d
Sponsors
Ajinomoto Co., Inc.
University of Toronto
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
10 Years to 15 Years
Healthy volunteers
Yes
Inclusion criteria
(Adolescents): * Healthy, active adolescent who regularly participates in moderate to vigorous physical activity 5 times a week for a minimum of 1 hour. * Being -0.5 to +1.0 years from peak height velocity, determined by the ratio of standing to sitting height, with the maximal age for adolescents being 16 years. * Obtaining a minimum level of 8.3 on the Beep Test
Exclusion criteria
(All Subjects): * Inability to meet health and physical activity guidelines according to the Physical activity readiness questionnaire (PAR-Q+) and Physical Activity Questionnaire, * an inability to adhere to any of the protocol guidelines (i.e. caffeine consumption) * a biological age falling outside of the aforementioned ranges
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| 13CO2 ([13]Carbon Dioxide) Excretion (µmol/kg/h) | 4 hours per study day | Each trial day will assess a different protein intake with 13CO2 excretion defining the level of protein adequacy. Each trial day will be separated by \ 1wk washout with all trial being completed in approximately 3 months. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| [13C]Phenylalanine Oxidation (µmol/kg/h) | 4 hours per study day | Each trial day will assess a different protein intake with 13CO2 excretion defining the level phenylalanine oxidation being used as a secondary marker of protein adequacy. Each trial day will be separated by \ 1wk washout with all trial being completed in approximately 3 months. |
Countries
Canada
Outcome results
None listed