HIV Infection
Conditions
Brief summary
This study evaluates the impact of highly active antiretroviral therapy on the size of the latent viral reservoirs in resting CD4+ T cells and monocytes in HIV positive patients. The activation state of the cells will be assessed, by measuring the activation of Akt, to determine its influence on the size of the viral reservoirs.
Interventions
Sponsors
Centre Hospitalier Universitaire de Besancon
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
No
Inclusion criteria
* HIV infected patients under Highly Active Anti-Retroviral Therapy (HAART) or Naïve from treatment
Exclusion criteria
* Age limits * Pregnancy * Legal restrictions to consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Measure of the integrated viral reservoir size | day 1 | Measure by Alu-PCR. Results are given in number of integrated proviral DNA copies per 1 million cells. |
| Measure of the proviral reservoir size | day 1 | Measure by Total Proviral HIV DNA detection kit. Results are given in number of total proviral DNA copies per 1 million cells. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Measure of Akt activation | day 1 | Measure of Akt activation in live cells by flow cytometry |
Countries
France
Outcome results
None listed